TABLE 3.

Oligonucleotides used for site-directed mutagenesisa

OligonucleotideSequence; description
M13-205′-GTAAAACGACGGCCAGTG-3′
hpaGL4A5′-ATGAATTCTGCGAACACACAGCTCG-3′; mutagenic primer used to construct HpaG(L4A)
hpaGQ7A5′-CTTTGAACACAGCGCTCGGCGC-3′; mutagenic primer used to construct HpaG(Q7A)
hpaGG9A5′-CACAGCTCGCCGCCAACTCGTC-3′; mutagenic primer used to construct HpaG(G9A)
hpaGF14D5′-CCAACTCGTCCGACTTTCAGGTTGAC-3′; mutagenic primer used to construct HpaG(F14D)
hpaGQ16A5′-TCCTTCTTTGCGGTTGACCCCG-3′; mutagenic primer used to construct HpaG(Q16A)
hpaGP19A5′-CAGGTTGACGCCGGCCAGAAC-3′; mutagenic primer used to construct HpaG(P19A)
hpaGG20E5′-GTTGACCCCGAACAGAACACGC-3′; mutagenic primer used to construct HpaG(G20E)
hpaGQ21A5′-GACCCCGGCGCGAACACGC-3′; mutagenic primer used to construct HpaG(Q21A)
hpaGQ24A5′-CCAGAACACGGCATCTAGTCCGAAC-3′; mutagenic primer used to construct HpaG(Q24A)
hpaGQ29A5′-AGTCCGAACGCGGGCAACCAG-3′; mutagenic primer used to construct HpaG(Q29A)
hpaGG30E5′-CCGAACCAGGAAAACCAGGGCATC-3′; mutagenic primer used to construct HpaG(G30E)
hpaGQ32A5′-CAGGGCAACGCGGGCATCTC-3′; mutagenic primer used to construct HpaG(Q32A)
hpaGG33A5′-CAGGGCAACCAGGCCATCTCGG-3′; mutagenic primer used to construct HpaG(G33A)
hpaGL39P5′-TCGGAAAAGCAACCGGACCAGC-3′; mutagenic primer used to construct HpaG(L39P)
hpaGL42D5′-AACTGGACCAGGACCTGACCCAGCTCATC-3′; mutagenic primer used to construct HpaG(L42D)
hpaGL50P5′-CAGCTCATCATGGCACCGCTGCAGCAG-3′; mutagenic primer used to construct HpaG(L50P)
hpaGD99A5′-CGTCGGAGCCATTCTCCAG-3′; mutagenic primer used to construct HpaG(D99A)
xopAF48L5′-GACCCAGCTCATCTTTTCAATG-3′; mutagenic primer used to construct XopA(F48L)
xopAM52L5′-CTTTTCACTGCTTCTGCAGG-3′; mutagenic primer used to construct XopA(M52L)
  • a Base differences from the wild-type sequence are shown in boldface italic.