TABLE 2.

Oligonucleotides used for deletion mutagenesis of hpaGa

OligonucleotideSequence; description
T7 promoter5′-TAATACGACTCACTATAGGG-3′
hpaGΔC775′-GGATCCTAATTGTTGCTCTGCTGAAG-3′; mutagenic primer used to construct HpaGΔC77
hpaGΔC795′-GGATCCTAGCTCTGCTGAAGCAGG-3′; mutagenic primer used to construct HpaGΔC79
hpaGΔC815′-GGATCCTACTGAAGCAGGGCCATG-3′; mutagenic primer used to construct HpaGΔC81
hpaGΔC835′-GGATCCTACAGTGCCATGATGAGCTGGGTC-3′; mutagenic primer used to construct HpaGΔC83
hpaGΔN6715′-CATATGGACTCTGGCGGTC-3′; mutagenic primer used to construct HpaGΔN67
hpaGrev5′-GGATCCTTACTGCATCGATC-3′; mutagenic primer used to construct HpaGΔN67
  • a Base differences from the wild-type sequence are shown in bold face italic. New BamHI and NdeI sites are indicated by underlined bases.