TABLE 1.

Bacterial strains and plasmids

Strain or plasmidCharacteristicsaSource or reference
Strains
    Escherichia coli
        DH5αF φ80dlacZΔM15 Δ(lacZYA-argF)U169 endA1 deoR recA1 hsdR17(rK mK+) phoA supE44 λ thi-1 gyrA96 relA1Gibco-BRL
        BL21(DE3)F ompT hsdSB(rB mB) gal dcm (DE3)Novagen
    Xanthomonas campestris pv. vesicatoria
        82-8Wild type, race 1R. E. Stall
        E-3Wild type, race 2R. E. Stall
        LS833Wild type, race 3S. Lee
Plasmids
    pT7-7T7 promoter-based expression vector; Ampr24
    pET14bT7 promoter-based expression vector; AmprNovagen
    pLysSEncoding T7 lysozyme gene; CmrNovagen
    pBluescript II SK(+)Phagemid, pUC derivative; AmprStratagene
    pBSNpBluescript II SK(+) with NdeI site in β-galactosidase gene start codonThis study
    pBHPAG0.4-kb NdeI-BamHI fragment, including entire hpaG gene, cloned into pBSNThis study
    pHpaGMTSmaI-BssHII fragment (T7 promoter region) of pBHPAG was deleted and religated; template for site-directed mutagenesis of hpaGThis study
    pTJ1PCR-amplified hpaG in pT7-7; AmprThis study
    pJ14hpaG in pET14b; AmprThis study
    pHGM61hpaG(L4A) in pET14b; AmprThis study
    pHGM62hpaG(Q7A) in pET14b; AmprThis study
    pHGM63hpaG(G9A) in pET14b; AmprThis study
    pHGM64hpaG(F14D) in pET14b; AmprThis study
    pHGM65hpaG(Q16A) in pET14b; AmprThis study
    pHGM66hpaG(P19A) in pET14b; AmprThis study
    pHGM67hpaG(G20E) in pET14b; AmprThis study
    pHGM68hpaG(Q21A) in pET14b; AmprThis study
    pHGM69hpaG(Q24A) in pET14b; AmprThis study
    pHGM70hpaG(Q29A) in pET14b; AmprThis study
    pHGM71hpaG(G30E) in pET14b; AmprThis study
    pHGM72hpaG(Q32A) in pET14b; AmprThis study
    pHGM73hpaG(G33A) in pET14b; AmprThis study
    pHGM26hpaG(S35A) in pET14b; AmprThis study
    pHGM7hpaG(E36A) in pET14b; AmprThis study
    pHGM27hpaG(K37A) in pET14b; AmprThis study
    pHGM28hpaG(Q38A) in pET14b; AmprThis study
    pHGM46hpaG(L39A) in pET14b; AmprThis study
    pHGM74hpaG(L39P) in pET14b; AmprThis study
    pHGM6hpaG(D40A) in pET14b; AmprThis study
    pHGM29hpaG(Q41A) in pET14b; AmprThis study
    pHGM46hpaG(L42A) in pET14b; AmprThis study
    pHGM75hpaG(L42D) in pET14b; AmprThis study
    pHGM36hpaG(L43P) in pET14b; AmprThis study
    pHGM30hpaG(T44A) in pET14b; AmprThis study
    pHGM40hpaG(Q45A) in pET14b; AmprThis study
    pHGM32hpaG(L46A) in pET14b; AmprThis study
    pHGM41hpaG(I47A) in pET14b; AmprThis study
    pHGM11hpaG(L50A) in pET14b; AmprThis study
    pHGM76hpaG(L50P) in pET14b; AmprThis study
    pHGM12hpaG(L51A) in pET14b; AmprThis study
    pHGM13hpaG(Q52A) in pET14b; AmprThis study
    pHGM42hpaG(Q53A) in pET14b; AmprThis study
    pHGM15hpaG(S54A) in pET14b; AmprThis study
    pHGM16hpaG(N55A) in pET14b; AmprThis study
    pHGM18hpaG(E58A) in pET14b; AmprThis study
    pHGM8hpaG(Q59A) in pET14b; AmprThis study
    pHGM19hpaG(G60V) in pET14b; AmprThis study
    pHGM9hpaG(Q61A) in pET14b; AmprThis study
    pHGM20hpaG(G62V) in pET14b; AmprThis study
    pHGM21hpaG(Q63A) in pET14b; AmprThis study
    pHGM38hpaG(G64A) in pET14b; AmprThis study
    pHGM10hpaG(Q65A) in pET14b; AmprThis study
    pHGM22hpaG(G66A) in pET14b; AmprThis study
    pHGM23hpaG(G67V) in pET14b; AmprThis study
    pHGM5hpaG(D99A) in pET14b; AmprThis study
    pHGM17hpaG(I120T) in pET14b; AmprThis study
    pHGM37hpaG(L121P) in pET14b; AmprThis study
    pHGM2hpaG(A126T) in pET14b; AmprThis study
    pHGM34hpaG(A126V) in pET14b; AmprThis study
    pHGM3hpaG(S131L) in pET14b; AmprThis study
    pHGM77hpaG(L43P/L50P) in pET14b; AmprThis study
    pTHGChpaG(ΔN67) in pT7-7; AmprThis study
    pTHG67hpaG(ΔC66) in pET14b; AmprThis study
    pTHG58hpaG(ΔC75) in pET14b; AmprThis study
    pTHG56hpaG(ΔC77) in pET14b; AmprThis study
    pTHG54hpaG(ΔC79) in pET14b; AmprThis study
    pTHG52hpaG(ΔC81) in pET14b; AmprThis study
    pTHG50hpaG(ΔC83) in pET14b; AmprThis study
    pTXOPAPCR product of xopA cloned into pET14b; AmprThis study
    pTXH2120-bp NdeI-PvuII fragment of pTXOPA and 73-bp PvuII-BamHI fragment of pTHG67 coligated into pET14b; AmprThis study
    pTXM1xopA(F48L) in pET14b; AmprThis study
    pTXM2xopA(M52L) in pET14b; AmprThis study
    pTXM4xopA(F48L/M52L) in pET14b; AmprThis study
  • a Ampr, ampicillin resistance; Cmr, chloramphenicol resistance.