TABLE 2.

Plasmids used in this study

PlasmidDescriptionSource or reference
pDG148spac expression vector; Ampr KanrW. L. Nicholson
pBESTpGEM4 containing the neomycin resistance cassette under control of repU promoter of B. subtilis13
pMPRYB146mutL ORF cloned into the SalI/XbaI sites of pDG148This studya
pMPRYB147mutS ORF cloned into the SalI/XbaI sites of pDG148This study
pMPRYB155mutSL ORF cloned into the SalI/XbaI sites of pDG148This study
pMPRYB1384,777-bp PCR fragment of mutSL operon ligated into SmaI-treated pUC19This study
pMPRYB140pMPR138 with a 2.5-kbp SmaI/PmeI internal deletion of the mutSL operon containing the neomycin cassette of pBEST released with SmaIThis study
  • a See Materials and Methods for description of construction.