TABLE 1.

Strains and plasmids used for this study

Strain or plasmidRelevant genotypeSource or reference
Strains
    JM109Used as wild typeStratagene
    GSO105JM109 ompR101 zhf37::Tn10This study
    BW25113Used as wild type 8
    SC200BW25113 ΔmicC::kan (Kanr)This study
    SC201BW25113 ompC-luc (Cmr)This study
    SC204BW25113 ompC-lucΔmicC::kan (Kanr Cmr)This study
    SC209BW25113 ompCmutant-luc (Cmr)This study
    SC210BW25113 ompCmutant-luc ΔmicC::kan (Kanr Cmr)This study
    SC216BW25113 ompCmutant-luc ΔmicC (Cmr)This study
    SC218BW25113 ompC-luc ΔmicC (Cmr)This study
    GSO107BW25113 hfq-1::Ω (Cmr)This study
Plasmids
    pUC119Cloning vector (Ampr)New England Biolabs
    pUC-micCpUC119 carrying ≈410-bp micC region (Ampr)This study
    pAlter-Ex2p15A origin cloning vector (Tetr)Promega
    pAE-micCpAlter-Ex2 carrying ∼410-bp micC region (Tetr)This study
    pAE-micCmutantpAlter-Ex2 carrying ∼410-bp mutated micC region (Tetr)This study
    pSP-luc+Cloning vector with luciferase reporter gene (Ampr)Promega
    pSP-ompC ompC mRNA fused to luc in pSP-luc+ (Ampr)This study
    pSP-ompCmutantMutated ompC mRNA fused to luc in pSP-luc+ (Ampr)This study
    pSP-ompC (cat) ompC mRNA fused to luc in pSP-luc+(Cmr)This study
    pSP-ompCmutant (cat)Mutated ompC mRNA fused to luc in pSP-luc+ (Cmr)This study
    pKD3Plasmid carrying cat gene cassette and oriRγ origin (Cmr) 8
    pKD4Plasmid carrying kan gene cassette and oriRγ origin (Kanr) 8
    pKD46Red recombinase expression plasmid (Ampr) 8
    pCP20FLP recombinase expression plasmid (Ampr) 8
    pGEM-2Cloning vector (Ampr)Promega
    pGEM-micCpGEM-2 carrying micC gene (Ampr)This study
    pGEM-micCmutantpGEM-2 carrying micC mutant gene (Ampr)This study
    pGEM-ompCpGEM-2 carrying ompC gene (Ampr)This study
    pGEM-micFpGEM-2 carrying micF gene (Ampr) 36
    pSP64Cloning vector (Ampr)Promega
    pSP64-dsrApSP64 carrying dsrA gene (Ampr)This study