TABLE 2.

Restriction fragment analysesa

Restriction fragment (total size [kb])Fragment size (kb)
SpeI (1,390)280, 240, 150, 140, 125, 100, 80, 80, 75, 40, 30, 25, 15, 10
PmeI (1,350)180, 150, 140, 130, 120, 110, 100, 90, 80, 75, 70, 50, 30, 25, 20, 15
  • a Fragments sizes were estimated by comparison with either molecular weight standard or known S. meliloti SpeI and PmeI restriction fragments.