Primers used for PCR and sequencinga
| Primer | Nucleotide sequence (5′-3′) | Position (bp)/gene/accession no./reference |
|---|---|---|
| VS174 | CATCTAGAGGAAGCAGTAGACGAAATCG | 19931-19950/L0097/AF125520 |
| VS175 | CATCTAGAGGTCTTCTTCCGGTTTGTTC | 24224-24205/L0105/AF125520 |
| VS177 | ATCCCGGGTGAGGCATAACCTGATTCGTG | 22700-22720/region between stx2 and L0105/AE125520 |
| VS178 | ATCCCGGGTTCATATACAGGTGTTCCTTTTGG | 21466-21443/region between argO and stx2/AE125520 |
| VS244 | ACGTCTAGATGAGCAAGATGCTGAATCACTG | 2688-2667/Z0443/AE005214 |
| VS245 | CGAGTCGACCATTCACCACCCTGAATTGAC | 1174-1194/lacI-Z0442 intergenic region AE005214 |
| VS246 | CGAGTCGACAGAGATTACAAAGTTGAATCGTCAG | 6257-6231/lacA/AE005213 |
| VS247 | ACGTCTAGAACTGTTACAGATGATCCCACTGC | 4685-4706/cynS/AE005213 |
| VS256 | CAGTCTAGACAGGTAAGGTTTATCTG | 15241-15221/escD/AE071034 |
| VS257 | CAGGTCGACTATATACCTTTGATAATTTTC | 13742-13765/sepL-espA intergenic/region AE071034 |
| VS264 | CAGGTCGACGCAACATCCGTTGTTAATGTTGAG | 13726-13704espA/AE071034 |
| VS265 | CAGTCTAGATCCCGGCAGAACGAATACCGTTC | 12256-12278/espD/AE071034 |
| VS266 | CAGGTCGACAATTTCACACAGGATACAGCTATG | 11304-11287lacZ/AE005213 |
| VS268 | CAAATTGCCACTCACCATTCAG | 11100-11121/lacZ/AE005213 |
| VS269 | TTGAATTATGGCCCACACCAG | 8451-8431/lacZ/AE005213 |
| VS270 | TGTATTGTAACAGTGGCCCGAAG | 7854-7876/lacY/AE005213 |
| VS275 | CCGGTTATTTACCAAGGGATATTGC | 13159-13183/espA/AE071034 |
| VS276 | AAATGGCGCCTCTTTACTTGACTG | 14266-14242/sepL/AE071034 |
| VS280 | CAGGTCGACCCTGATAAGCGAAGCGTATCAGGC | 6191-6214/cynX-lacA intergenic region/AE005213 |
| VS303 | CATCTAGAGATTCCGTTCTCCGTTATGC | 3045-3026/acrB/AE005213/AE005225 |
| VS304 | CAGTCGACTCGGACATACTTCTACCCATG | 1630-1650/ybaJ-hha intergenic region/AE005225 |
| VS305 | CAGTCGACACTACTGAACAACATAAAGGTG | 1771-1792/ybaJ/AE005225 |
| VS306 | CAGTCGACTCGCTTTCGGAGCTATAACCG | 1409-1388/ylaD-hha intergenic region/AE005225 |
| VS307 | CATCTAGAGCTGGCAGGAGATAAGGAGGT | 1-20/downstream of ylaC/AE005225 |
| VS308 | ACGGATCCATGGTATTAATATGCAGGATC | 1769-1746/hha/AE005225 |
| VS309 | CAGGATCCCCGGTTATAGCTCCGAAAG | 1389-1409/hha/AE005225 |
| VS317 | ACGTCGACCCGGTTATAGCTCCGAAAG | 1389-1409/hha/AE005225 |
| VS318 | ACGAATTCAATGTCCGAAAAACCTTTAACG | 1637-1619/hha/AE005225 |
| VS319 | CCGTTGAAGTGAAAGACGGTC | 1453-1474/gapA/AE005401 |
| VS320 | AACCACTTTCTTCGCACCAGC | 1638-1618/gapA/AE005401 |
| VS323 | TGTGGATGCCAAAATTGCTG | 13493-13474/espA/AE071034 |
| VS324 | CGCCTTCACTGTTTGCAGATC | 13322-13342/espA/AE071034 |
| VS327 | CATCTAGAACAGGCTCTAAAACGGTTAAC | 41697-41677/LEE/AE071034 |
| VS328 | CAGTCGACGCTTTAATATTTTAAGCTATTAGCG | 40200-40222/LEE/AE071034 |
| VS330 | CATCTAGACTTATCCTCTGGTATGATATCTTC | 38596-38619/LEE/AE071034 |
| VS331 | CAGGATCCATGCGGAGATTATTTATTATG | 40199-40173/lerORF/AE071034 |
| VS332 | CAGTCGACATGTTAAATATTTTTCAGCGG | 39806-39827/lerORF/AE071034 |
| VS339 | AAAGACATGATATCGGCACAGC | 2018-1918/ybaJ/AE005225 |
| VS340 | TGAGCATATTGCGACCTTCG | 1882-1864/ybaJ/AE005225 |
| VS341 | CGGAATTCGTCCAGATGACA | 8153-8168/hilApromoter/AE008831.1/14 |
| VS342 | TGGGGTGTAAATGCTGCTT | 8627-8609/hilApromoter/AE008831.1/14 |
| VS353 | GCTTAACCACCTCATCCTTCG | 13639-13659/espApromoter/AE071034 |
| VS354 | GACTGGTTGCTGGAGAGAGTCC | 13982-13963/espApromoter/AE071034 |
| VS355 | GAATGTATGGACTTGTTGTATGTG | 40139-40162/lerpromoter/AE071034 |
| VS356 | TTTGTTTATGCAATGAGATCTATC | 40526-40505/lerpromoter/AE071034 |
| VS376 | AGTCCATCATCAGGCACATTAG | 39985-40006/lerORF/AE071034 |
| VS371 | CAGGATCCATGAAGAAGATGTTTATGGCGG | 22433-22454/stx2B/AF125520 |
| VS372 | CAGTCGACTCAGTCATTATTAAACTGCACTTCAG | 22702-22676/stx2B/AF125520 |
| VS377 | CATACAACAAGTCCCATACATTC | 40160-41139lerORF/AE071034 |
| VS398 | GAGTCGACCATGAAATAATTAAATGATAACG | 30809-39787/LEE/AE071034 |
| VS415 | GGAGTCGACGAATAAAAACCAAGCTGAACC | 40534-40554/LEE/AE071034 |
| VS426 | GCAGTTCCTTTGCTTCATTG | 39642-39662/LEE/AE071034 |
| VS427 | GCCTTCCTGTAACTCGAATTAAG | 40593-40571/LEE/AE071034 |
↵a The position of the primer sequence represents the location in the published sequence deposited under the indicated accession number at NCBI. Underlined sequences GGATCC, GAATTC, GTCGAC, CCCGGG, and TCTAGA represent restriction sites for BamHI, EcoRI, SalI, SmaI, and XbaI, respectively.