TABLE 1.

Estimation of the number of bacteria per spore of G. margaritaa

Spore setNo. of spores used per preparationEstimated no. of isolated bacteriaCalculated no. of bacteria/sporeMean (SD) of No. of bacteria/sporec
12,00025 × 10612,50012,500 (2,500)
2,0002 × 10710,000
1,00015 × 10615,000
21,00010710,000
310,00010810,000
422,00035 × 10716,000
515,00015 × 10710,000
615,0002 × 10813,500
75009 × 106b18,00019,000 (1,414)
500107b20,000
850013 × 106b26,000
95009 × 106b18,00018,670 (1,155)
500107b20,000
5009 × 106b18,000
1040,00025 × 1076,300
1140,00035 × 1078,800
121,0004 × 1064,0003,690 (470)
1,0003 × 1063,000
1,0004 × 1064,000
8003 × 1063,750
  • a Bacteria were counted manually when conventional fluorescence microscopy was used or counted with ImagePro Plus software when confocal microscopy was used. The data are rounded numbers.

  • b Bacterial counts obtained when confocal microscopy was used.

  • c Means shown are for spore set series 1, 7, 9, and 12.