TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant featuresReference or source
V. harveyi strains
    BB120Wild type1
    BB170luxN::Tn52
    BB721luxO::Tn54
    BB886luxPQ::Tn53
    JAF375luxN::Cmr luxQ::Kanr12
    JAF483luxOD47A linked to Kanr12
    JAF536luxU::Tn513
    JAF548luxOD47E linked to Kanr12
    JAF633ΔluxM linked to Kanr14
    KM387ΔluxSThis study
    KM357luxR::Tn518
    KM413ΔluxS ΔluxMThis study
    JMH597luxN::Tn5 cqsS::CmrThis study
    JMH598cqsS::CmrThis study
    JMH603cqsA::CmrThis study
    JMH605ΔluxM linked to Kanr; cqsA::CmrThis study
    JMH606ΔluxS cqsA::CmrThis study
    JMH612luxPQ::Tn5 cqsS::CmrThis study
    JMH621luxU::Tn5 cqsA::CmrThis study
    JMH625ΔluxN luxQ::Tn5This study
    JMH626ΔluxN luxQ::Tn5 cqsA::CmrThis study
    JMH628ΔluxN luxQ::Tn5 cqsS::CmrThis study
    JMH634ΔluxM ΔluxS cqsA::CmrThis study
    JMH670ΔluxM ΔluxS cqsA::Cmr vhpA::Tn5lacZThis study
    JMH674ΔluxM ΔluxS cqsA::Cmr vcrD::Tn5lacZThis study
    JMH763cqsAS::CmrThis study
    JMH774luxOD47E cqsA::CmrThis study
V. cholerae strains
    KSK1052ΔluxS25
    MM893ΔcqsA25
Plasmids
    pLAFR2Broad-host-range cosmid; mob, Tetr15
    pRK2013Broad host range, tra, Kanr9
    pPHIJ1Broad host range, tra, mob, Gmr5
    pGEM-TCloning vector; AmprPromega
    pALTER AmpAmprPromega
    pALTER TetCloning vector; TetrPromega
    pBluescript SKCloning vector; AmprStratagene
    pKD3PCR template for Wanner deletions; Cmr8
    pCP20Ts FLP recombinase plasmid; Ampr, Cmr8
    pKAS46R6K replication origin; Smr33
    pBB1pLAFR with V. harveyi luxCDABE locus
    pBB154pLAFR with luxM and luxN locus2
    pMM467Tetr pALTER with cqsAThis study
    pKM556pLAFR2 with ΔluxM26
    pKM755pLAFR2 with vhpA::Tn5lacZ KanrThis study
    pJMH17F7pLAFR2 with cqsAVh and cqsSVh locus; TetrThis study
    pJMH25pLAFR2 with type III secretion locus; Tetr18
    pJMH159pJMH17F7 cqsAVh::Tn5lacZ KanrThis study
    pJMH163pJMH17F7 cqsAVh::Tn5lacZ KanrThis study
    pJMH164pJMH17F7 cqsAVh::Tn5lacZ KanrThis study
    pJMH240pJMH17F7 with cqsAVh::CmrThis study
    pJMH244pJMH17F7 with cqsSVh::CmrThis study
    pJMH253pGEM-T with cqsAVhThis study
    pJMH273pLAFR with cqsAVhThis study
    pJMH280pGEM-T with cqsSVhThis study
    pJMH282pLAFR with cqsSVhThis study
    pJMH300pBB154 with ΔluxNThis study
    pJMH325pJMH25 with vcrD::Tn5lacZ KanrThis study
    pJMH422pJMH17F7 with cqsASVh::CmrThis study