TABLE 2.

Inhibition of PhaZ1-dependent nPHB hydrolysis by detergents, specific hydrolase inhibitors, and redox active agents, and influence of ions

AgentaRelative activityb (%) at the following concn:
Detergentsc0.0001%0.001%0.05%0.1%
    Tween 20726103
    Tween 8057543
    Triton X-100805533
    SDS9889452
Ions1 mM5 mM10 mM100 mM
    MgCl21407540NP
    CaCl21307535NP
    NaCl14013087
    KCl14013075
Chelator1 mM5 mM10 mM20 mM
    EDTA79482421
    EDTA + CaCl2 (2.5 mM)88
    EDTA + MgCl2 (2.5 mM)86
Serine hydrolase inhibitors0.01 mM0.1 mM1 mM10 mM
    DFP49109
    PMSF (corrected by effect of 2-propanol)>999489
    DDSC (corrected by effect of 2-propanol)>999898
    2-propanol (vol/vol)91866246
Redox active agents1 mM4 mM
    2-Mercaptoethanol1411
    DTE166
    DTT200
    DTNB9692
    DTT + DTNB (equimolar)37
  • a DTE, 1,4-dithioerythritol; DTNB, dithionitrobenzoate.

  • b —, the value has not been determined; NP, precipitation was observed, but determination of activity was not possible.

  • c For Tween 20, Tween 80, and Triton X-100, concentrations are expressed in percentages (volume/volume); for SDS, concentrations are expressed as percentages (weight/volume).