TABLE 1.

Bacterial strains and plasmidsa

Strain or plasmidRelevant genotype and descriptionSource or reference
E. coli
    EHEC 86-24stx2+ EHEC strain (serotype O157:H7)18
    EHEC Δstx286-24 deleted of stx2This study
    EHEC Δstx2 Δlac86-24 deleted of stx2 and lac operonThis study
    EHEC esp::lac86-24 Δstx2 Δlac containing chromosomal esp::lac transcriptional fusionThis study
    EHEC esp::lac/TnKnTransposon mutant of EHEC esp::lacThis study
    EHEC esp::lac hha::TnKn86-24 esp::lac containing transposon TnKn in hhaThis study
    EHEC esp::lac Δhha86-24 esp::lac deleted of hhaThis study
    EHEC Δhha86-24 deleted of hhaThis study
    TOP 10endA1 recA1 hsdR17(rK mK) supE44 φ80dlacZΔM15Δ(lacZYA-argF)Invitrogen
    DH10BendA1 recA1 hsdR17(rK mK) supE44 φ80dlacZΔM15Δ(lacZYA-argF)Gibco-BRL
    BL21F ompT hsdS(rB mB) gal dcmAmersham Biosciences
Plasmids
    pCR2.1Cloning vectorInvitrogen
    pBR322Cloning vector4
    pAM450Suicide vector27
    pUC18Cloning vector45
    pGEX-4T-3Protein expression vectorAmersham Biosciences
    pSM71pAM450 derivative used for deleting stx2 in EHEC 86-24This study
    pSM80pAM450 derivative used for deleting the lac operon in 86-24 Δstx2This study
    pSM102pAM450 derivative used for constructing an esp::lac transcriptional fusionThis study
    pSM116pBR322 containing a cloned copy of hhaThis study
    pSM122pAM450 derivative used for deleting hhaThis study
    pSM138pGEX-4T-3 carrying an hha ORF for producing GST-Hha fusion proteinThis study
    pSM142pCR2.1 containing esp promoterThis study
    pSM143pCR2.1 containing hilA promoterThis study
    pSM146pCR2.1 containing ler promoterThis study
    pSM163pGEX-4T-3 carrying ler ORF for producing GST-Ler fusion proteinThis study
    pSM188pAM450 derivative used for deleting lerThis study
    pSM197RpCR2.1 containing hhaThis study
    pSM212pAM450 derivative used for constructing an esp::lac transcriptional fusionThis study
  • a Detailed descriptions of the bacterial strains and plasmids listed in this table are provided in Materials and Methods.