TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionaReference or source
S. gordonii strains
    M99Parent strain 21
    PS624M99 Δgly::spc; Spcr 24
    PS649M99 Δnss::spc; Spcr 24
    PS666M99 Δgtf::spc; Spcr 24
    PS668M99 Δorf4::spc; Spcr 24
    PS768M99(pGST-GspBS1-BR)This study
    PS769M99(pGST-GspBBR)This study
    PS771PS624(pGST-GspBS1-BR)This study
    PS772PS649(pGST-GspBS1-BR)This study
    PS773PS666(pGST-GspBS1-BR)This study
    PS774PS668(pGST-GspBS1-BR)This study
    PS780PS624(pGST-GspBBR)This study
    PS781PS649(pGST-GspBBR)This study
    PS782PS666(pGST-GspBBR)This study
    PS783PS668(pGST-GspBBR)This study
    PS806M99(pGST-GspBS1-BR-S2)This study
    PS807PS624(pGST-GspBS1-BR-S2)This study
    PS808PS649(pGST-GspBS1-BR-S2)This study
    PS809PS666(pGST-GspBS1-BR-S2)This study
    PS810PS668(pGST-GspBS1-BR-S2)This study
    PS811M99(pGST-GspBBR-S2)This study
    PS812PS624(pGST-GspBBR-S2)This study
    PS813PS649(pGST-GspBBR-S2)This study
    PS814PS666(pGST-GspBBR-S2)This study
    PS815PS668(pGST-GspBBR-S2)This study
    PS826M99(pGST-GspBS2S)This study
    PS827PS624(pGST-GspBS2S)This study
    PS828PS649(pGST-GspBS2S)This study
    PS829PS666(pGST-GspBS2S)This study
    PS830PS668(pGST-GspBS2S)This study
    PS831M99(pGST-GspBS2L)This study
    PS832PS624(pGST-GspBS2L)This study
    PS833PS649(pGST-GspBS2L)This study
    PS834PS666(pGST-GspBS2L)This study
    PS835PS668(pGST-GspBS2L)This study
    PS868M99(pMSP3545)This study
E. coli strains
    DH5αHost for cloning vectors 15
    BL21Host for pGEX-3X derivativesNovagen
    PS873BL21(pGEX-3X)This study
    PS874BL21(pGEX-GspBS1-BR)This study
    PS875BL21(pGEX-GspBS1-BR-G-O4)This study
    PS876BL21(pGEX-GspBS1-BR-ΔG-O4)This study
    PS877BL21(pGEX-GspBS1-BR-G-ΔO4)This study
Plasmids
    pGEX-3XpBR322 replication origin, gst laclq Ptac, MCS; AmprAmersham Biosciences
    pUC19ColE1 replication origin, lacZ′, MCS; Ampr 26
    pMSP3545pAMβ1 and ColE1 replication origins, nisRK PnisA, MCS; Ermr 5
    pGST-GspBS1-BR-S2pMSP3545 carrying gst and a fragment of gspB spanning SRR1, the basic region, and part of SRR2This study
    pGST-GspBBRpMSP3545 carrying gst and a fragment of gspB which spans the basic regionThis study
    pGST-GspBS1-BRpMSP3545 carrying gst and a fragment of gspB which spans SRR1 and the basic regionThis study
    pGST-GspBBR-S2pMSP3545 carrying gst and a fragment of gspB which spans the basic region and part of SRR2This study
    pGST-GspBS2LpMSP3545 carrying gst and a fragment of gspB which spans part of SRR2This study
    pGST-GspBS2SpMSP3545 carrying gst and a fragment of gspB which spans part of SRR2This study
    pGEX-GspBBRpGEX-3X carrying a fragment of gspB which spans the basic regionThis study
    pGEX-GspBS1-BRpGEX-3X carrying a fragment of gspB which spans SRR1 and the basic regionThis study
    pGEX-GspBS1-BR-G-O4pGEX-GspBS1-BR carrying both gtf and orf4This study
    pGEX-GspBS1-BR-ΔG-O4pGEX-GspBS1-BR-G-O4 with an in-frame deletion of the gtf coding regionThis study
    pGEX-GspBS1-BR-G-ΔO4pGEX-GspBS1-BR-G-O4 with an in-frame deletion of the orf4 coding regionThis study
  • a Spcr, spectinomycin resistant; Ampr, ampicillin resistant; Ermr, erythromycin resistant; MCS, multiple cloning site; SRR1, first serine-rich region; SRR2, second serine-rich region.