TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Source or referencea
Strains
    B. megateriumSource of PHBDSMZ32
    B. cereusSource of PHBDSMZ31
    C. violaceumSource of PHV, PHASCLDSMZ30191
    E. coli JM109Cloning strain 52
    E. coli DH5αCloning strain 14
    E. coli S17-1Mobilizing strain 43
    E. coli HMS174Host for phaP and/or phaCBA 22
    P. oleovoransSource of PHAMCLATCC 29347
    P. putida KT224Source of PHAMCL
    R. rubrum S1Wild typeDSMZ467
    R. rubrum SmRifStreptomycin- and rifampin-resistant mutant of S1 17
    W. eutropha H16Source of PHBDSMZ428
Plasmids
    pJoe890Stem-loop cloning vectorGift from J. Altenbuchner, Stuttgart, Germany
    pJoe4036Rhamnose-inducible vector for His-tagging 47
    pECFPecfpBD Clontech
    pBBR1-MCS2Broad-host-range vector 25
    pJM9238 phaCBA 22
    pBluescript KS(−)Cloning vectorStratagene
    pBluescript KS::phaP phaP 22
    pSN2213pBluescript KS(−)::2.1-kbp SalI::phaZ1This study
    pSN2384pBBR1-MCS2::phaZ1-ecfpThis study
    pSN2388pBBR1-MCS2::Plac-phaZ1-ecfpThis study
    pSN2552pBBR1-MCS2::phaZ1-3x-gly-ecfpThis study
    pSN2564pBBR1-MCS2::Plac-phaZ1-3x-gly-ecfpThis study
    pSN2558pBBR1-MCS2::phaZ1-6x-gly-ecfpThis study
    pSN2565pBBR1-MCS2::Plac-phaZ1-6x-gly-ecfpThis study
  • a DSMZ, Deutsche Sammlung für Mikroorganismen und Zellkulturen GmbH.