TABLE 5.

Distribution of PHB depolymerase (PhaZ1), MDH, and AP activities in cell fractions of R. rubrum

FractionActivity (U [%]) of:
PhaZ1MDHAP
Cell-free culture supernatant<50 (<3)5.5 (5)<35 (<3)
Supernatant of lysozyme-EDTA treatment1,600 (88)24 (20)1,100 (93)
Ultrasonic extract of pellet after lysozyme-EDTA treatment210 (12)88 (75)78 (7)