TABLE 1.

Purification of the PduO adenosyltransferase used in this study

Purification stepTotal protein (mg)Sp act. (nmol min−1 mg−1)Total activity (nmol min−1)Yield (%)Purification (fold)
Inclusion body isolation12019423,2801001
Solubilization with CHES12024829,7601281.3
Phenyl Sepharose chromatographya202625,240231.4
  • a In the phenyl sepharose chromatography step, only the fractions of highest purity were pooled and used for further study. Typically about 80 to 90% of the total activity was recovered from the phenyl Sepharose column, but the majority of the eluate contained a minor contaminant and was discarded.