TABLE 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionSource or reference
E. coli ER 2566F λ fhuA2[lon] ompT lacZ::T7 genel gal sulAII Δ(mcrC-mrr)114::IS10 R(mcr-73::miniTn10)2 R(zgb-210::Tn10)1 (TeTS) endA1 [dem]6
Plasmids
    pK18Kmr, derivative of cloning vector pUC1826
    pK19Kmr, derivative of cloning vector pUC1926
    pTYB1Apr, T7 RNA polymerase-based expression vectorNew England Biolabs
    pGEM-7Zf(+/−)AprPromega
    PCR-ScriptSK(+)AprStratagene
    pK18FIIS4.4Kmr, pK18 with a 4.4-kb SalI fragment containing R. capsulatus cbbRill, cbbF, and part of cbbP24
    pK18FIIB2.3Kmr, pK18 with a 2.3-kb BamHI fragment containing R. capsulatus cbbF and part of cbbP and cbbRill24
    pEULA4Kmr, pK19 with a 4-kb EcoRI fragment containing R. capsulatus cbbL, cbbRI, part of anfA, and uncharacterized sequence between cbbRI and anfA23
    pEULPE1.2Kmr, pK19 with a 1.2-kb PstI-HindIII fragment containing R. capsulatus cbbRI-cbbI promoterG. C. Paoli and F. R. Tabita (unpublished data)
    RIscript6Apr, PCR script with an amplified fragment containing cbbRI from the first to the last codons plus NdeI site at the 5′- end and SapI site at the 3′ end43
    pTYBRIApr, pTYB1 with an NdeI-SapI fragment from pRIscript643
    pGEM-7RIIApr, pGEM-7 with an amplified fragment containing cbbRII from the first to the last codons plus NdeI site at the 5′ -end and the SapI site at the 3′ end43
    pTYBRIIApr, pTYB1 with an NdeI-SapI fragment from pGEM-7RII43