TABLE 1.

Strains, plasmids, and primers used in this study and their relevant characteristics

Strain, plasmid, or primerRelevant featuresReference
L. plantarum     strains
    WCFS1Wild type whose genome sequence is available19
    MD007L. plantarum WCFS1 Δalr, d-alanine auxotroph23
Plasmids
    pNZ7120Emr, pIL252 derivative containing Lactococcus lactis alr promoter probe5
    BI1 to BI96PNZ7120 derivatives containing chromosomal L. plantarum fragments that were initially identified as inducible by 0.1% porcine bileThis study
Primers
    PB15′-ACCGCTACGGATCACATC-3′5
    PB25′-CTCGGGGCAGTAAGACTA-3′5
    PB35′-GTGGTGAAGTTTTCATGG-3′5
    16S-fo25′-TGATCCTGGCTCAGGACGAA-3′This study
    16S-re25′-TGCAAGCACCAATCAATACCA-3′This study
    237-fo5′-CTACTGATATGGTTGTCGGGAATTA-3′This study
    237-re5′-ACGGGTGCGTAGAAGAAGC-3′This study
    775-fo5′-GCTCTTGCACCGGATATCAA-3′This study
    775-re5′-TTTCTTCTTCCCGTGACCAGT-3′This study
    1898-fo5′-GTGGCGACGGTTCTTACCAT-3′This study
    1898-re5′-CCCTGGAAGACCAATCGTGT-3′This study
    1027-fo5′-CCATGATGGTGCTTCACAA-3′This study
    1027-re5′-TCGTGGCAGCAGAGGTAATG-3′This study