TABLE 2.

Adenosyltransferase activities of truncated versions of PduOa

ProteinSize (amino acids)Approx mol mass (Da)Adenosyltransferase activity (nmol min−1 mg−1)kcat (min−1)
PduO1-901-909,908NDbND
PduO1-1851-18520,8894339.04
PduO1-2301-23025,7603669.42
PduO1-2931-29332,4892849.23
PduO1-33636,8122629.64
  • a PduO and its truncated versions were partially purified as described in Materials and Methods.

  • b ND, not detectable under conditions used.