TABLE 2.

Analysis of biofilm development in wild-type and Pf4-expressing P. aeruginosa strains with the COMSTAT softwarea

ParameterDayWild typeWild type + Pf4SCV7
Mean biomass (μm−3 μ−2)10.43 ± 0.090.30 ± 0.020.47 ± 0.13
30.74 ± 0.070.94 ± 0.131.19 ± 0.55
71.87 ± 0.072.48 ± 0.575.29 ± 0.62
Mean thickness (μm)10.36 ± 0.090.32 ± 0.030.53 ± 0.14
30.82 ± 0.061.01 ± 0.111.41 ± 0.65
72.02 ± 0.033.08 ± 0.896.37 ± 0.93
Maximum thickness (μm)111.40 ± 1.5311.9 ± 0.3341.60 ± 1.60
311.66 ± 0.2115.2 ± 0.0243.20 ± 9.73
712.80 ± 0.3219.2 ± 0.8957.60 ± 7.82
Mean microcolony area at19.73 ± 2.935.28 ± 2.36123.49 ± 51.62
substratum (μm2)35.76 ± 5.758.11 ± 3.61181.31 ± 81.62
717.00 ± 2.9526.93 ± 4.64294.70 ± 146.22
  • a The values are means ± standard errors for data from 15 image stacks (five image stacks from three replicate biofilms) Boldface type indicates values that are significantly higher than the values for the wild-type strain as determined by analysis of variance.