TABLE 2.

Plasmids used in this study

PlasmidDescriptiona
p3TETSuicide vector for insertional mutagenesis, Tetr
pML01p3TET derivative containing a 903-bp internal fragment of rr01 (XbaI/SphI)
pML02p3TET derivative containing a 486-bp internal fragment of rr02 (BanII/AccI)
pML03p3TET derivative containing a 551-bp internal fragment of rr03 (AvaII)
pML04p3TET derivative containing a 567-bp internal fragment of rr04 (EcoRI*/BamHI*)
pML05p3TET derivative containing a 540-bp internal fragment of rr05 (Bsp1061)
pML06p3TET derivative containing a 568-bp internal fragment of rr06 (SfaNI)
pML07p3TET derivative containing a 556-bp internal fragment of rr07 (Sau3AI/RsaI)
pML08p3TET derivative containing a 476-bp internal fragment of rr08 (EcoRI*/BamHI*)
pML09p3TET derivative containing a 518-bp internal fragment of rr09 (BspHI/PmlI)
pML10p3TET derivative containing a 537-bp internal fragment of rr10 (RsaI)
pML11p3TET derivative containing a 520-bp internal fragment of rr11 (EcoRI/NdeI)
pML12p3TET derivative containing a 575-bp internal fragment of rr12 (Sau3AI)
pML13p3TET derivative containing a 483-bp internal fragment of rr13 (DraI/HinFI)
pML14p3TET derivative containing a 499-bp internal fragment of rr14 (AccI/XbaI)
pML15p3TET derivative containing a 622-bp internal fragment of rr15 (HincII/RsaI)
pML16p3TET derivative containing a 465-bp internal fragment of rr16 (HincII)
pML17p3TET derivative containing a 460-bp internal fragment of rr17 (BsmAI/BsiWI)
pML18p3TET derivative containing a 554-bp internal fragment of rr18 (SfaNI)
pML19p3TET derivative containing a 1,408-bp fragment encompassing 5′ region of hk02, intergenic region, and 3′ region of an open reading frame encoding a putative lipoprotein (EcoRI/SphI)
  • a *, sites artificially introduced by oligonucleotides used in PCR.