Plasmid | Description^{a} |
---|---|

p3TET | Suicide vector for insertional mutagenesis, Tet^{r} |

pML01 | p3TET derivative containing a 903-bp internal fragment of rr01 (XbaI/SphI) |

pML02 | p3TET derivative containing a 486-bp internal fragment of rr02 (BanII/AccI) |

pML03 | p3TET derivative containing a 551-bp internal fragment of rr03 (AvaII) |

pML04 | p3TET derivative containing a 567-bp internal fragment of rr04 (EcoRI*/BamHI*) |

pML05 | p3TET derivative containing a 540-bp internal fragment of rr05 (Bsp1061) |

pML06 | p3TET derivative containing a 568-bp internal fragment of rr06 (SfaNI) |

pML07 | p3TET derivative containing a 556-bp internal fragment of rr07 (Sau3AI/RsaI) |

pML08 | p3TET derivative containing a 476-bp internal fragment of rr08 (EcoRI*/BamHI*) |

pML09 | p3TET derivative containing a 518-bp internal fragment of rr09 (BspHI/PmlI) |

pML10 | p3TET derivative containing a 537-bp internal fragment of rr10 (RsaI) |

pML11 | p3TET derivative containing a 520-bp internal fragment of rr11 (EcoRI/NdeI) |

pML12 | p3TET derivative containing a 575-bp internal fragment of rr12 (Sau3AI) |

pML13 | p3TET derivative containing a 483-bp internal fragment of rr13 (DraI/HinFI) |

pML14 | p3TET derivative containing a 499-bp internal fragment of rr14 (AccI/XbaI) |

pML15 | p3TET derivative containing a 622-bp internal fragment of rr15 (HincII/RsaI) |

pML16 | p3TET derivative containing a 465-bp internal fragment of rr16 (HincII) |

pML17 | p3TET derivative containing a 460-bp internal fragment of rr17 (BsmAI/BsiWI) |

pML18 | p3TET derivative containing a 554-bp internal fragment of rr18 (SfaNI) |

pML19 | p3TET derivative containing a 1,408-bp fragment encompassing 5′ region of hk02, intergenic region, and 3′ region of an open reading frame encoding a putative lipoprotein (EcoRI/SphI) |

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*a**, sites artificially introduced by oligonucleotides used in PCR.