TABLE 3.

Phenotypic and competitive virulence analysis of SMIT mutants

StrainDisrupted gene or homologApparent auxotropyCIa
In vitroIn vivo
IVT15lysCLysine0.840.32
IVT24saeS0.360.15
IVT27ORF0.310.11
IVT29ORF0.820.32
IVT35ydiF0.940.40
IVT36tyrATyrosine0.790.28
IVT40narJ0.700.67
IVT42yitL0.290.25
IVT59arlS0.250.10
IVT133yflS1.060.14
IVT171LMO25871.360.63
IVT173dapBLysine0.840.24
IVT176yuiF0.920.16
IVT207braBLysine0.840.29
IVT211yneP0.440.28
IVT252hipOLysine1.100.20
IVT285trpFTryptophan0.890.59
IVT291phoX0.920.67
IVT626aspBAspartate0.730.001
IVT627pycAAspartate0.910.02
IVT631gabP0.820.03
IVT636pyrCUracil0.340.19
IVT638asdLysine0.800.26
  • a CI determinations were relative to isogenic wild-type SAM23 (see Materials and Methods).