TABLE 2.

Attenuated S. aureus strains identified by SMIT

ClassificationStrainGene (organism)aLengthbInsertion sitecStrain N315 ORFPutative or known function
Biosynthesis of small moleculesIVT15lysC401196SA1225Aspartate kinase
IVT36tyrA (B. halodurans)366263SA1197Prephenate dehydrogenase
IVT173dapB230132SA1228Dihydrodipicolinate synthase
IVT252hipO383367SA1230Hippurate hydrolyase
IVT285trpF21153SA1203Phosphoribosylanthranilate isomerase
IVT626aspB (B. circulans)428154SA1749Aspartate aminotransferase
IVT627pycA (B. halodurans)1,073589SA0963Pyruvate carboxylase
IVT636pyrC (B. halodurans)425131SA1044Dihydroorotase
IVT638asd32991SA1226Aspartate semialdehyde dehydrogenase
Cell surface binding and transportIVT35vga642512SA1852Streptogramin resistance ABC-type transporter
IVT133yflS (B. subtilis)472159SA2486Predicted 2-oxoglutarate/malate translocator
IVT176yuiF (B. subtilis)43846SA0804Predicted permease
IVT207braB4482SA1239Branched-chain amino acid transporter
IVT291pstS (B. anthracis)32715SA1221Phosphate ABC transporter binding protein
IVT631gabP484400SA1169Gamma-aminobutyrate permease
Signal transductionIVT24saeS353278SA0660Regulator of exotoxin gene expression
IVT59arlS451401SA1246Regulator of extracellular proteases
IVT84arlS451118SA1246Regulator of extracellular proteases
EnergyIVT40narJ (S. carnosus)200151SA2183Anaerobic nitrate reductase, delta subunit
UnknownIVT27SE0526 (S. epidermidis)164161SA0699
IVT29SE1059 (S. epidermidis)255251SA1209
IVT42yitL (B. subtilis)300279SA1223
IVT171LMO2587 (L. monocytogenes)28292SA2478
IVT211yneP (B. subtilis)155113SA1185Predicted thioesterase
  • a Previously described S. aureus gene designation or name of the gene and source organism encoding the ORF with highest similarity.

  • b Gene ORF length (condons).

  • c Codon site of transposon insertion.