TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotypeReference
Strains
    MG1655Wild-type E. coli5
    MC1000Δ(ara-leu) Δlac rpsL1506
    MG1ΔrelBE derivative of MC10007
    EMG2Wild-type E. coli K-12 CGSC# 44018
    KL310ΔrelBEF derivative of EMG2This work
    KL312ΔhipBA derivative of EMG2This work
    AT984F+ dapA9
    HM21AT984 zde-264::Tn1010
    HM22AT984 zde-264::Tn10 hipA710
    KL313ΔhipBA derivative of HM22This work
Plasmids
    pKD13Template plasmid aphA1
    pKD46λ Red recombinase expression plasmid bla1
    pCP20pSC101rep (Ts) bla cat1
    pBAD33P15 cat araC pBAD11
    pKP219Mini-R1 bla lacIq pA1/O4/O312
    pKP3033pKP219 pA1/O4/O3::opSD::relB13
    pKP3035pBAD33 pBAD::relE13