TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotypeReference
Strains
    MG1655Wild-type E. coli 5
    MC1000Δ(ara-leu) Δlac rpsL150 6
    MG1ΔrelBE derivative of MC1000 7
    EMG2Wild-type E. coli K-12 CGSC# 4401 8
    KL310ΔrelBEF derivative of EMG2This work
    KL312ΔhipBA derivative of EMG2This work
    AT984F+dapA 9
    HM21AT984 zde-264::Tn10 10
    HM22AT984 zde-264::Tn10 hipA7 10
    KL313ΔhipBA derivative of HM22This work
Plasmids
    pKD13Template plasmid aphA 1
    pKD46λ Red recombinase expression plasmid bla 1
    pCP20pSC101rep (Ts) bla cat 1
    pBAD33P15 cat araC pBAD 11
    pKP219Mini-R1 bla lacIq pA1/O4/O3 12
    pKP3033pKP219 pA1/O4/O3::opSD::relB 13
    pKP3035pBAD33 pBAD::relE 13