TABLE 1.

Subset of selected genes showing a ≥3-fold change in expression under at least one growth condition

Functional categoryORFGene productAerobicAnaerobic
naMeanb (fur/WT)nMean (fur/WT)
Transport and binding proteins
    CationsSO0139Ferritin (fin)86.41109.2
SO0583Bacterioferritin-associated ferredoxin (bfd)1113.221028.61
SO0742Iron(III) ABC transporter; ATP-binding protein31.6245.99
SO0743Iron(III) ABC transporter; permease protein412.7743.29
SO0744Iron(III) ABC transporter; periplasmic iron(III)-binding protein90.9654.13
SO1111Bacterioferritin subunit 2 (bfr2)81.64110.16
SO1482TonB-dependent receptor; putative1026.811243.7
SO1580TonB-dependent heme receptor42.4866.36
SO1783Ferrous iron transport protein A (feoA)411.1975.04
SO1784Ferrous iron transport protein B (feoB)83.54127.49
SO3030Siderophore biosynthesis protein (alcA)107.931010.44
SO3031Siderophore biosynthesis protein; putative103.4183.46
SO3032Siderophore biosynthesis protein; putative88.4812.69
SO3033Ferric alcaligin siderophore receptor86.411013.49
SO3669heme transport protein (hugA)626.451074.96
SO3670TonB1 protein (tonB1)1019.021133.16
SO3671TonB system transport protein ExbB1 (exbB1)1038.661030.4
SO3672TonB system transport protein ExbD1 (exbD1)617.65734.82
SO3673Hemin ABC transporter; periplasmic hemin-binding protein (hmuT)98.83527.04
SO3674Hemin ABC transporter; permease protein (hmuU)84.58916.3
SO3675Hemin ABC transporter; ATP-binding protein (hmuV)87.831034.73
SO4516Ferric vibriobactin receptor (viuA)88.2168.77
SO4523Iron-regulated outer membrane virulence protein (irgA)65.611243.83
SO4743TonB-dependent receptor; putative1026.21236.23
SOA0153Heavy-metal efflux pump; CzcA family74.4571.3
PorinsSOA0114Outer membrane protein A (ompA)64.2971.07
Unknown substrateSO0450Major facilitator family protein125.19123.01
SO0821ABC transporter; ATP-binding/permease protein115.03122.14
SO0822Outer membrane efflux family protein83.11112.76
SO1865ABC transporter, ATP-binding protein110.3141.1
Energy metabolism
    Electron transportSO0264Cytochrome c (scyA)72.2940.19
SO2916Phosphate acetyltransferase (pta)80.3683.43
SO4062Polysulfide reductase; subunit A (psrA)80.03121.08
SO1778Decaheme cytochrome c (omcB)110.19120.25
SO1779Decaheme cytochrome c (omcA)110.17120.21
SO1019NADH dehydrogenase 1; C/D subunits (nuoCD)110.9280.22
SO1020NADH dehydrogenase 1; B subunit (nuoB)81.3350.18
SO0846Iron-sulfur cluster-binding protein NapH (napH)417.93101.41
SO0847Iron-sulfur cluster-binding protein NapG (napG)49.4691.05
SO0849NapD protein (napD)238.6980.82
SO0845Cytochrome c-type protein NapB (napB)60.07102.08
SO0848Periplasmic nitrate reductase (napA)312.5571.12
SO1782Decaheme cytochrome c MtrD (mtrD)43.941.51
SO1777Decaheme cytochrome c MtrA (mtrA)120.22120.37
SO3921Periplasmic Fe hydrogenase; small subunit (hydB)122.6350.09
SO3920Periplasmic Fe hydrogenase; large subunit (hydA)81.8470.15
SO2098Quinone-reactive Ni/Fe hydrogenase; large subunit (hyaB)20.2260.16
SO2099Quinone-reactive Ni/Fe hydrogenase; small subunit precursor (hoxK)120.2741.51
SO4591Tetraheme cytochrome c (cymA)120.7370.29
SO0809Azurin precursor (azu)410.2632.15
SO1364Iron-sulfur cluster-binding protein120.32120.58
SO1427Decaheme cytochrome c110.3100.47
SO2727Cytochrome c381.5150.12
SO3420Cytochrome c83.6570.42
  • a Number of replicate data points (out of a total of 12) included in the analysis.

  • b Relative gene expression is presented as the mean ratio of the fluorescence intensity of the fur deletion mutant (FUR2 strain) to that of the parental strain (WT).