TABLE 2.

Genes encoding hypothetical proteins that displayed a ≥5-fold change in expression in the FUR2 strain under at least one growth condition and identification of probable operons

ORFLocationaAerobicAnaerobic
nbMeanc (FUR2/WT)nMean (FUR2/WT)
SOA0051S0.4350.06
SOA0070S120.1470.52
SO0324S80.07120.92
SO0447dM (3)84.81810.41
SO0448dM (3)54.4576.37
SO0449dM (8)1210.371216.71
SO0786S25.7742.33
SO0792S336.22NAeN/A
SO0797fM (1)93.9793.77
SO0798fS1026.736103.09
SO0844S212.9240.85
SO0908S75.3452.82
SO0975M (8)80.2100.32
SO1450S4134.37313.51
SO1454S30.1740.56
SO1188gM (3)10165.71036.96
SO1189gM (1)1218.981129.51
SO1190gM (1)1215.471057.36
SO1572S36.26411.43
SO1658M (2)364.9142.57
SO1947M (3)80.1860.63
SO2039S82.87812.78
SO2425hS316.8643.64
SO2671S65.1730.58
SO2686iS1210.371216.71
SO2687iS1012.1541.21
SO2688iS106.3951.26
SO2689iM (1)68.97NAN/A
SO2691iS65.3840.792
SO2697iS127.5250.99
SO2699iS65.0931.022
SO2702iS1025.2260.04
SO2703iS28.35NAN/A
SO2735M (1)85.62101.74
SO2929S80.17110.83
SO2998S311.8149.64
SO3003S29.9343.17
SO3004S238.7551.16
SO3011S319.9821.07
SO3025S1118.8888.3
SO3062M (3)1055.82967.72
SO3344M (4)728.9726.15
SO3369S2176.2745.99
SO3406jM (3)72.45710.82
SO3407jM (8)1124.481127.73
SO3408jM (4)1121.19845.67
SO3587M (1)62.10811.16
SO3667kS859.661056.73
SO3668kS1037.51051.41
SO3676M (1)353.5663.83
SO3891M (1)20.0660.42
SO3913S62.2846.45
SO4185S39.0130.76
SO4700S118.6719.33
SO4740M (2)377.83913.15
  • a Predicted cellular location of the gene product, i.e., cytosol (soluble) (S) or membrane associated (M). The number given in parentheses next to the M designation refers to the number of transmembrane helices predicted using the computer program SOSUI (40).

  • b Number of replicate data points (out of a total of 12) included in the analysis.

  • c Relative gene expression is presented as the mean ratio of the fluorescence intensity of the FUR2 strain to that of the parental strain (WT).

  • d Probable operon SO0447-SO0448-SO0449.

  • e NA, not available.

  • f Probable operon SO0797-SO0798.

  • g Probable operon SO1188-SO1189-SO1190.

  • h Probable operon SO2426 (DNA-binding response regulator)-SO2425, SO2426 was up-regulated 30.2-fold and 25.9-fold under aerobic and anaerobic conditions, respectively.

  • i Probable operon SO2684 to SO2704.

  • j Probable operon SO3406-SO3407-SO3408.

  • k Probable operon SO3667-SO3668.