TABLE 4.

Quantification of AcmA-binding to L. lactis MG1363acmAΔ1 and L. lactis MG1363 (dltD acmAΔ1)a

Cells addedOD600 reductionBound AcmA activity (%)
No cells0.0530
MG1363acmAΔ10.03240
MG1363 (dltD acmAΔ1)0.03142
  • a The percentage of AcmA activity from 1-ml supernatant samples that bound to cells from 1-ml culture samples was determined as described in Materials and Methods. Samples containing AcmA were incubated with M. lysodeikticus cells for 30 min. Similar results were obtained in independent experiments.