TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristicsaSource or reference
P. aeruginosa strains
    PAO1Wild type 17
    PDO100 rhlI::Hgr PAO1 derivative 2
    YL101PAO1 with an rhlA-gfp fusion inserted in attBThis study
    YL100PDO100 with an rhlA-gfp fusion inserted in attBThis study
    YL120PAO1 with chromosomal aracC-Pbad-gfp fusion (stable gfp) on the chromosome in attBThis study
E. coli strains
    DH5αF′/endA1 hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF) U169 deoR [φ80 dlacZ-M15 recA1] 41
    SM10Kmr, thi-1 thr leu tonA lacY supE recA::RP4-2Tc::Mu 10
Plasmids
    pUC19Ampr, E. coli cloning vector 42
    pUCP18Cbr, pUCP18 vector containing a P. aeruginosa origin of replication 32
    pPROBE-ATGFP translational fusion vector with P. aeruginosa and E. coli replication origins 24
    Mini-CTX-lacZTetr, integration-proficient vector for chromosomal insertion at the attB site 1
    pFLP2Amprflp recombinase plasmid 14
    pRHLI-2Gmr, pBBR1MCS5 with ptac-rhlI 26
    pUO103Kmr Smr, pJRD215 containing rhlI 25
    pYL120pUC19 with an rhlAB promoter fragmentThis study
    pYL121Ampr, rhlAB-gfp transcription fusion in pPROBE-ATThis study
    pYL122Ampr, rhlA-gfp transcription fusion in mini-CTX-lacZThis study
    pYL125pUCP18 with rhlI geneThis study
    pYL126pUCP18 with ptac-rhlIThis study
    pYL182Ampr, araC-Pbad-gfp transcription fusion in pPROBE-ATThis study
    PYL183Gmr, araC-Pbad-gfp transcription fusion in mini-CTX-gmThis study
    pmRFP1DsRed in pRESTB 4
  • a Hgr, mercury resistance; Kmr, kanamycin resistance; Ampr, ampicillin resistance; Cbr, carbanicillin resistance; Tetr, tetracycline resistance; Gmr, gentamicin resistance.