TABLE 1.

Predicted genes of bacteriophage ES18

GeneaStart codonStop codonSD (spacer)AAscPredicted functionBest matches (phage/gene)% Identity/ no. of AAs
1ATGTGAAA (4)143Small subunit terminasePasteurella prophage Past1/PM177749/133
Haemophilus prophage φFlu/HI141155/81
2ATGTAGAGGAG (12)439Large subunit terminasePhotorhabdus prophage/plu290178/442
Sf6/233/223
APSE-1/1831/225
SPP1/226/423
3TTGTGAAGGG (8)88UnknownNone
4GTGTAAGAGGA (6)52UnknownNone
5ATGTAAGGA (6)449Portal proteinActinobacillus prophage/Aple10900133/441
Haemophilus prophage φFlu/HI140919/357
6ATGTAAGGAG (5)308Procapsid assemblyD3112/2823/152
Mu/3017/161
7ATGTAAGGAGA (7)421UnknownActinobacillus prophage/Aple10920130/448
8ATGTAAGGAGA (6)149Decoration?None
9ATGTAAAAGGA (9)358Coat?None
10ATGTAGAAGGA (6)62UnknownNone
11ATQTAAAGAGG (5)133UnknownNone
12ATGTGAGGGGA (6)59UnknownNone
13ATGTGAGGAGG (6)120UnknownActinobacillus prophage/Aple10970130/120
14ATGTGAGAGG (5)131UnknownNone
15ATGTAAAAGGA (8)128UnknownSp5/ECs121036/36
stx2φI/p15336/36
16ATGTAAGGAGA (7)245Tail shaftPY54/1346/88
Felix-01/7950/86
HK97/1034/94
17ATGTAAGGGG (4)217UnknownActinobacillus prophage/Aple11010126/219
18ATGTAGGGA (8)57UnknownNone
19ATGTAGGGGAA (7)509Unknown; moronPasteurella prophage/orf536/461
EJ-1/0332/485
Streptococcus prophage φ8382.5/180640/291
20ATGTAAGGGA (7)151Unknown; moron?Bordetella prophage Bbr1/BB170532/85
21ATGTAGGGAGA (7)1,124Tape measureCP-933P/Z603427/809
HK97/1428/768
22ATGTGAAGG (4)132Unknown; moron?None
23ATGTAAGGAGG (5)115Tail tipGifsy-1/STM259338/111
lambda/M32/114
24ATGTAAGGGAGA (4)86Unknown; moronE. coli 933R prophage/Z237233/69
25ATGTAAAGGAG (5)95Unknown; moronNone
26GTGTGAGGGAG (6)234Tail tipGifsy-2/STM104547/215
lambda/L43/215
27ATGTGAAGAAGG (7)239Tail tipFels-1/STM092245/236
lambda/K49/198
28ATGTAAGAGG (8)115Tail tiplambda/I40/57
Gifsy-2/STM104734/95
29ATGTGAAAGG (7)1,051Tail tip fiber (host range)lambda/J40/847
Gifsy-1/STM258939/846
N15/2132/1,069
30ATGTAAGGGGG (7)104UnknownHK022/2443/102
N15/2246/102
31ATGTAAGGAGA (7)217UnknownN15/2333/203
HK022/2534/201
32ATGTGAAGGGA (5)80Superinfection exclusion (N15 cor)N15/24 (cor)62/74
HK022/2661/78
33ATGTGAAGGAG (5)378Tail fiber?N15/2573/161
φYe03-12/1725/234
34ATGTAAAGAGGA (5)387IntegraseP22/int96/387
SfV/int88/386
35ATGTGAGGAG (4)116ExcisionaseP22/xis96/115
SfV/2785/115
36GTGTAAGGAGG (6)118UnknownP22/eaA69/99
Fels-1/STM089567/49
37ATGTGAGAGGAA (10)186UnknownST64T/eaa175/68
φ4795/p0484/51
38ATGTAAGGAGG (7)126Unknownε15/4685/68
39ATGTAAGGGAG (4)72Unknownε15/4788/72
933W/L00750/72
40ATGTGAGAGGA (6)81UnknownNone
41ATGTAAAGGAG (8)274UnknownP22/eaD68/154
ε15/4568/154
42ATGTGAAGGGG (7)121UnknownPY54/4854/93
43ATGTGAAGGA (7)208UnknownP22/eaE93/56
ε15/4442/208
44ATGTAAGAGAG (6)56UnknownP22/orf5692/56
Sf6/234/51
45ATGTGAGAGGA (6)95UnknownSf6/2466/95
P2/orf8339/91
46TTGTGAAAG (9)105RecombinationP22/abc278/97
HK97/3977/97
47ATGTAAGG (9)165UnknownT7/7.742/131
48ATGTAAAGG (8)156Single-strand DNA binding proteinE. coli/ssb90/153
Sf6/2789/157
49ATGTAAGAGG (6)235Recombination (P22 erf)ST646T/orf23596/235
Sf6/2891/235
50ATGTGAGGAGG (5)62UnknownST64T/orf6698/66
HK620/hkaM66/62
51ATGTGAAGGAG (8)37Inhibition of host septationST64T/kil100/37
lambda/kil58/36
52ATGTGAAGGAG (6)28Inhibition of host HflB proteaselambda/cIII85/27
HK620/hkaO85/27
53ATGTGAAGGAA (7)282UnknownXp10/4429/149
54ATGTAAGAGGA (7)109Transcription antiterminationST64T/2476/108
P22/2485/75
55ATGTAAGATGA (7)216Repressor (lambda cI)P22/c2100/216
434/cI51/207
56ATGTAAAAAG (12)61Repressor (lambda cro)P22/cro100/61
21/cro98/61
57ATGTGAAGGAA (9)92Transcription activationP22/cI100/92
(lambda cII)Sf6/4184/92
58ATGTAAAGGAG (5)53UnknownHK97/5394/53
Sf6/4294/53
59ATGTGAAGGAG (6)282Replication, ori recognitionlambda/O93/193
P22/1842/150
60ATGTGAAGG (6)35UnknownNone
61ATGTGAGAGG (5)626Primase-helicaseFlex3/SF068295/371
T7/425/355
62ATGTGAGAG (6)92UnknownNone
63ATGTGAAGGAG (8)26UnknownP22/ninA100/25
Sf6/45100/25
64ATGTGAAGAGG (5)145UnknownP22/ninB97/145
lambda/ninB97/145
65ATGTAAGGAGA (6)57UnknownP22/ninD98/57
lambda/ninD73/57
66ATGTGAAGGAGG (4)58UnknownST64T/ninE78/55
lambda/ninE76/55
67ATGTAGGAGGAG (6)60UnknownHK620/hkbB252/57
HK97/64 (ninF)50/57
68ATGTAAGGAGG (6)72UnknownNone
69ATGTGAGGAG (6)201UnknownP22/ninG94/201
lambda/ninG89/203
70ATGTGAGAGG (5)74UnknownP22/ninY100/74
71ATGTAAGGGAGG (4)67UnknownP22/ninH98/67
lambda/ninH71/57
72ATGTGAAGAGG (5)59UnknownST64T/ninZ92/57
P22/ninZ89/57
73ATGTGAGGGAGG (9)254Transcription antiterminationE. coli prophage CP-933M/Z134545/274
(lambda Q)SfV/4641/257
74ATGTAAGAGG (6)108Lysis timing, holin inhibitorP22/13100/108
lambda/S89/106
75ATGTAAGAA (4)105Lysis, holinP22/13′100/106
lambda/S′89/104
76ATGTGAGAGG (6)145Lysis, lysozymeP22/R97/146
Gifsy-1/STM261251/150
77GTGTAAAGAGCG (11)156Lysis (lambda Rz)Fels-1/STM090885/145
lambda/Rz69/155
78ATGTGAAGGA (8)62Lysis (lambda Rz1)lambda/Rz173/60
933W/Rz170/60
79ATGTAAAGA (8)229Affects growth in IHFb hostHaemophilus prophage φFlu/HI141234/95
P22/orf201 (rha)24/121
  • a Arrow indicates direction of transcription.

  • b IHF defective.

  • C AA, amino acid.