TABLE 3.

Purification of RokA protein

Purification stepTotal protein (mg)Total activity (U)Sp act (U/mg)Yield (%)Fold purifi- cation
Streptomycin sulfate- treated cell extract588 (441)a410.3 (307.7)b0.931001
DEAE28.7 (9.7)a334.9 (113.2)b34.53837.2
Hydroxyapatite3.7 (1.3)a62.4 (21.4)b481951.6
Mono-Q0.292068.69373.9
  • a Numbers in parentheses indicate the amount of protein used for the next purification step.

  • b Numbers in parentheses indicate the total activity used for the next purification step.