TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype or descriptionSource or reference
Bacterial strain
    E. coli
        DH5α-λpirφ80dlacZΔM15 Δ(lacZYA-argF)U196 recA1 hsdR17 deoR thi-1 supE44 gyrA96 relA1pir 23a
        S17-1-λpirthi pro recA hsdR [RP4-2Tc::Mu-Km::Tn7pir Tpr Smr 36a
    S. oneidensis
        MR-1Wild type 41a
        AS93MR-1 tagged with eGFP in a Tn7 construct, Cmr Gmr 40
        AS95Plasmid integration mutant of flhB in AS93, nonmotile, Cmr Gmr Kmr 40
        AS124In-frame deletion of arcA in AS93, Cmr GmrThis study
        AS123In-frame deletion of crp in AS93, Cmr GmrThis study
        AS122In-frame deletion of etrA in As93, Cmr GmrThis study
        AS127AS124 harboring pME6031::arcA, Cmr Gmr TcrThis study
        AS125AS123 harboring pME6031::crp, Cmr Gmr TcrThis study
        AS126AS122 harboring pME6031::etrA, Cmr Gmr TcrThis study
Plasmid
    pBluescript KS+Apr ori ColE1New England Biolabs
    pGP704-Sac28-KmKmr mobRP4+ ori-R6K sacB, suicide plasmid for in-frame deletionsChengyen Wu, unpublished data
    pGP704-Sac28-Km::FarcIn-frame deletion fragment of arcA in pGP704-Sac28-KmThis study
    pGP704-Sac28-Km::FcrpIn-frame deletion fragment of crp in pGP704-Sac28-KmThis study
    pGP704-Sac28-Km::FetrIn-frame deletion fragment of etrA in pGP704-Sac28-KmThis study
    pME6031repA oriVpVS1 oriVp15A oriT Tcr 17a
    pME6031::arcAarcA in pME6031This study
    pME6031::crpcrp in pME6031This study
    pME6031::etrAetrA in pME6031This study