TABLE 5.

Km and Vmax values of highly purified RokA kinase activity with various substrates

SubstrateKm (mM)Vmax (μmol/min/mg)
Glucose0.5869
NAG0.8537
Mannose1.7550
2-Deoxyglucose1.821.4
Fructose44.52.9
ATP0.23a69a
manNAccNDbND
NANAcND ND
maltosecND ND
galactosecND ND
  • a Values of Km and Vmax for ATP were measured with 6.7 mM glucose.

  • b ND, not detected.

  • c Concentration of sugars tested to measure the kinase activities was >50 mM.