TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant genotype or descriptionReference or source
B. fragilis
    ADB77TM4000; ΔthyA Tpr 3
    CJB100ADB77:ΔrokA1This work
    CJB101ADB77:ΔrokA1ΔhexA1This work
    CJB200ADB77:ΔhexA1This work
    ATCC 25285Subclone of NCTC 9343 26
    TAL2480Cefr 12
    TAL3636Cefr 12
    ATCC 23745 rokA + hexA + 26
    TAL21616-21633Clinical isolates acquired from Tufts Anaerobic LaboratoryThis work
B. thetaiotaomicron
    VPI 8582 rokA + hexA + 45
E. coli
    DH5αλ nonlysogen 44
    HB101 rpsL20 38
Plasmid
    RK231RP4 derivative, Tetr, Tra+ 17
    pYT102p15A ori, Cmr, RP4 oriT, B. fragilis suicide vector containing B. fragilis thyA+ Tetr 37
    pJST61E. coli-B. fragilis shuttle vector, Ampr, Clnr 38
    pKDel12pYT102 ΔrokA1This work
    pHDe12pYT102 ΔhexA1This work
    pBFROK10pJST61 with 2.4-kb fragment from TM4000 containing the rokA geneThis work
    pBFROK40pJST61 with 1-kb fragment from TM4000 containing the rokA gene under the λ PL promoterThis work
    pBFHEX10pJST61 with 1.5-kbp fragment from TM4000 containing the hexA geneThis work
    pBFROKHISpJST61 with 1.1-kb fragment from TM4000 containing the rokA gene with an oligohistidine- tagged C terminusThis work