TABLE 3.

High- and low-threshold genes in the Spo0A regulona

Category and geneTranscriptional ratioFunction
PΔv/Δ0Awt/PΔv
High-threshold activated
    spoIIG1.215.9pro-σE processing protease/σE factor
    spoIIE1.014.3Ser phosphatase (σF activation)/asymmetric septum formation
    racA0.89.2Remodeling and anchoring of the chromosome
    spoIIA2.06.8Anti-anti-σF/anti/σFF
    yneE1.86.2Unknown
    yttP1.13.0Similar to transcriptional regulator (TetR/AcrR family)
    sinI1.62.6Antagonist of SinR (transcriptional regulator)
High-threshold repressed
    rapA4.90.2Asp phosphatase (dephosphorylation of Spo0F-P)
    flgB0.70.3fla/che operon: motility and chemotaxis
    ftsE0.90.3Cell division protein (ATP binding)
    lytE0.60.3Cell wall hydrolase
    divIVA1.00.3Cell division protein
    yfmI19.20.3Similar to macrolide efflux transporter
    yxbC51.30.4Unknown
    sdp50.10.4Sporulation delaying protein
    dltA1.10.4d-Alanyl-d-alanine carrier protein ligase
    rocD0.50.4Omithine aminotransferase
Low-threshold activated
    yxbC51.30.4Unknown
    sdp50.10.4Sporulation delaying protein
    yfmI19.20.3Similar to macrolide efflux transporter
    yqcG9.00.5Unknown
    yybN9.00.5Unknown
    skf8.21.0Sporulation killing factor
    yjcM5.70.5Unknown
    rapA4.90.2Asp phosphatase (dephosphorylation of Spo0F-P)
    spo0A3.51.1Transcriptional master regulator for sporulation
    yqxI3.50.6Unknown
    kinA2.50.6Sensor histidine kinase (initiation of sporulation)
    ykzF2.50.7Unknown
    spo0F2.50.5Response regulator (multicomponent P-relay)
Low-threshold repressed
    abrB0.20.6Transcriptional pleiotropic regulator of transition stage genes
    fruR0.3NDTranscriptional repressor of the fructose operon
    yqzD0.40.6Unknown
    ykaA0.40.8Unknown
    purT0.41.0Phosphoribosylglycinamide formyltransferase 2
    med0.41.1Positive regulator of comK
Not differentially expressed
    accD0.81.2Acetyl coenzyme carboxylase (beta subunit)
    dnaA0.80.7Initiation of chromosome replication
    dnaG1.30.6DNA primase
    holB0.90.5DNA polymerase III (delta′ subunit)
    kinC1.80.8Sensor histidine kinase
    metS0.91.3Methionyl-tRNA synthetase
    nfrA1.11.8FMN-containing NADPH-linked nitro/flavin reductase
    relA1.10.6GTP pyrophosphokinase (stringent response)
    rok1.30.6Repressor of comK
    soj1.10.5Negative regulation of sporulation initiation
    tkt0.50.7Transketolase
    yaaD0.90.5Unknown
    yerB0.81.0Unknown
    ycgM0.62.0Similar to proline oxidase
    ylmD1.60.7Unknown
    yocH1.41.6Similar to cell wall-binding protein
    yppD1.61.6Unknown
    yrrL0.70.9Similar to folate metabolism
    yusE1.00.8Unknown
    yvyE0.60.5Unknown
σK regulated
    cotDK)0.5NDSpore coat protein (inner)
Read-through from yuzC (σE)
    yuxH0.213.3Unknown
  • a Not differentially expressed, <2-fold difference for activation and <0.5-fold difference for repression; ND, signal intensity not significantly above background level; wt, wild type level.