TABLE 1.

Bacterial strains and plasmids

Strain or plasmidDescriptionSource or reference
E. coli strains
    CC118(λpir)CC118 lysogenized with λpir phage 18
    TG1Host for DNA manipulations 42
    HB101 supE44 hsdS20(rB mB) recA13 ara-14 proA2 lacY1 galK2 rpsL20 xyl-5 mtl-1 42
P. aeruginosa strains
    PAO1-VClinical isolateV. de Lorenzo
    JFL30MexAB-OprM-hyperexpressing MDR mutant from PAO1-VPresent work
    JFL28MexCD-OprJ-hyperexpressing MDR mutant from PAO1-VPresent work
    JFL10MexXY-hyperexpressing MDR mutant from PAO1-VPresent work
    PAO1-LClinical isolateA. Lazdunski
    JFL05MexEF-OprN-hyperexpressing MDR mutant from PAO1-LPresent work
    R-1 lasR mutant derived from PAO1-LA. Lazdunski
    PT462 rhlR mutant derived from PAO1-LT. Kohler
Plasmids
    pGEMT-EasyApr; vector for cloning of PCR productsPromega
    pVLT35Smr; RSF1010-lacIq/Ptac hybrid broad-host-range expression vector, multiple cloning site of pUC18 14
    pJF03pVLT35 derivative plasmid that carries the wild-type exsA gene from P. aeruginosa PAO1Present work
    pRK600CmroriColE1 RK2 Tra+, helper plasmid in triparental mating 23