TABLE 1.

Strains and plasmids

Strain or plasmidRelevant characteristicsSource or reference
P. aeruginosa strains
    PA14Wild type63
    SMC241Wild type/pSMC21This study
    SMC416sad-110 (pilA)::Tn5B21(Tcr, ′lacZ)This study
    SMC257sad-36 (flgK)::Tn5B30(Tc)56
    SMC693sad-160::Tn5B21(Tcr, ′lacZ)This study
    SMC707sad-160::Tn5B21(Tcr, ′lacZ)/pSMC21This study
    SMC1022ΔsadRS::Gmr (ΔPA3946-PA3947)This study
    SMC1023ΔsadRS::Gmr/pSMC21This study
    SMC1368ΔsadS (ΔPA3946)This study
    SMC1437ΔsadS/pSMC21This study
    SMC1061ΔsadR (ΔPA3947)This study
    SMC1101ΔsadR/pSMC21This study
    SMC1259ΔsadA (ΔPA3948)This study
    SMC1263ΔsadA/pSMC21This study
    MID383pscI::TnphoAF. Ausubela
    MID241pcrD::TnphoAF. Ausubela
    PAO1Wild type35
    MID33913pcrV::ISphoA/hah35
    MID21792pcrH::ISphoA/hah35
    MID20540popB::ISphoA/hah35
Plasmids
    pACΩGMGmr65
    pCVD442Apr, sacB, suicide vector for generating chromosomal knockout mutations18
    pEXGWGateway modified pEX18GmMatt Wolfgang
    pEX18GmGmr, sacB, suicide vector for generating chromosomal knockout mutations30
    pSU21Cmr, cloning vector47
    pSMC21Apr, Kmr, GFP expression vectorThis study
    pSMC32Cloning vector, KmrThis study
    pSMC42KO plasmid for ΔsadRS::Gmr, in pCVD442This study
    pSMC44KO plasmid for ΔsadR, in pEXGWThis study
    pSMC57KO construct for ΔsadA in pGEM-TThis study
    pSMC58KO construct for ΔsadS in pSU21This study
    pSMC59KO plasmid for ΔsadA, in pEX18GmThis study
    pSMC65KO plasmid for ΔsadS, in pEX18GmThis study
    pGEM-T easy vectorApr, cloning vectorPromega, Inc.