TABLE 1.

Strains and plasmids used in this study

Strain or plasmidGenotype or relevant characteristicsSource or reference
HK207HK205 dsbB C41Y21
HK209HK205 dsbB R48C21
HK211HK205 dsbB R48H21
JCB816MC1000 dsb+ phoR λ malF-lacZ102 zih-12::Tn10Lab collection
JCB819MC1000 dsbB::kan-5 phoR λ malF-lacZ102 zih-12::Tn10Lab collection
JT272MG1655, pCLL2100This work
JT357JT272, dsbA CPPC zih-12::Tn10This work
JT358JT272, dsbA CSFC zih-12::Tn10This work
JT359JT272, dsbA, CPSC zih-12::Tn10This work
JT360JT272, dsbA CPLC zih-12::Tn10This work
JT368JT272, yihF::insAB_5This work
JT427MG1655 dsbA M153R, pCLL2100This work
JT491MG1655, pCLL2100 non-dsbA or -dsbB mutationThis work
JT503JT272, yihE::yi22_6This work
JT656BL21 dsbA::kan-5, pJT656This work
JT720JT491, dsbB/pBAD33This work
JT844JT503, yihE/pBAD33This work
JT855JT491, dsbA/pBAD33This work
JT890JT503, λdsbA+This work
MB31D10, pMS421, pBJ41Lab collection
PB406SF100 dsbA CPPC8
PB410SF100 dsbA CSFC8
PB416SF100 dsbA CPSC8
PB419SF100 dsbA CPLC8
VL1jJCB819, pACYC184-lacIq-tet, pWM76 R48AThis work
VL2jJCB819, pACYC184-lacIq-tet, pWM76 Q95AThis work
VL3jJCB819, pACYC184-lacIq-tet, pWM76 Q33AThis work
VL4jJCB819, pACYC184-lacIq-tet, pQE70This work
VL5jJCB819, pACYC184-lacIq-tet, pWM76This work
VL6jJCB819, pACYC184-lacIq-tet, pWM76 H91AThis work
VL14jJCB819, pACYC184-lacIq-tet, pWM76 Y46FThis work
VL15jJCB819, pACYC184-lacIq-tet, pWM76 R48KThis work
pBJ41pKK233-2 dsbA5
pCLL2100pCLL2300 ccrA29
pJT651pWM76 dsbB L43PThis work
pJT653pWM76 dsbB R133CThis work
pJT655pWM76 dsbB L146RThis work
pJT656pET11a dsbA M153RThis work
pVL1pWM76 dsbB R48AThis work
pVL2pWM76 dsbB Q95AThis work
pVL3pWM76 dsbB Q33AThis work
pVL6pWM76 dsbB H91AThis work
pVL14pWM76 dsbB Y46FThis work
pVL15pWM76 dsbB R48KThis work
pWM76pQE70 dsbB C8A, C49V1
λD69λ bam λ 1° Δ(sr1-sr2) imm-21 nin-5 shn-6O5
λdsbA+2.5-kb HindIII from p12-7 cloned into λD695