TABLE 2.

Coiled-coil mutational analyses and coiled-coil predictions

Coiled-coil designation or amino acid positionaMutant designationAmino acid sequence or repeat motif patternbHeptad repeat position of the residue mutatedfactor H bindingcCOILS analysis result
Window = 21Window = 28
cc1 abcdefgabcdefgabcdefgabcdefga
aa 73-101A68wt ASELKAIGKELEDQKKEENIQIAKIAKEK ++0.9080.32
1m1 .....................K..S.... a,d0.5110.091
1m2 .....................V..V.... a,d++0.7480.138
cc2 abcdefgabcdefgabcdefgabcdefg
aa 132-159A68wt LDYKKENIEKLKEILEILKKNSEHYNII ++0.9960.997
2m1 ....R.....S...H............. e,d,a0.0440.039
2m2 ..............H..H.......... a,d0.2420.139
2m3 ...........E.T.............. e,g++1.001.00
2m4 ..........V...V............. d,a+/−0.5370.588
2m5 ...............K..E.......... b,e+0.9970.998
cc3 abcdefgabcdefgabcdefgabcdefg
aa 197-224A68wt LMQIKSNLEIKQRLKKTLNETLKVYNQN ++0.7080.979
3am1 Q..K........................ a,d0.7080.129
3am2 ...K...S.................... d,a+/−0.1030.100
3am3 .K..E....................... b,e++0.8300.998
3bm1 .................S...H...... d,a+/−0.0350.010
3bm2 ..................D.A....... e,g++0.5420.95
  • a cc, coiled coil; aa, amino acid.

  • b Repeat motif patterns are given in lowercase. Dots indicate residues that are identical to the A68wt sequence.

  • c ++, strong binding; −, no binding; +/−, weak binding.