TABLE 3.

Effect of different metals on iron depletion of E. coli strains harboring multiple gene deletionsa

StrainGenotypeSystems still functionalGrowth yield in the presence of DIP andb:
No metalMgCl2ZnCl2MnCl2FeCl3
GR499ΔzupT::cat ΔmntH ΔfeoABC ΔentCFec0.070 ± 0.010.09 ± 0.040.14 ± 0.020.64 ± 0.020.66 ± 0.08
GR507ΔzupT::cat ΔfeoABC ΔentCFec, MntH0.39 ± 0.010.31 ± 0.010.51 ± 0.090.69 ± 0.030.65 ± 0.01
GR489ΔmntH::cat ΔfeoABC ΔentCFec, ZupT0.45 ± 0.030.42 ± 0.010.42 ± 0.010.64 ± 0.010.69 ± 0.08
GR460ΔfeoABC::cat ΔentCFec, ZupT, MntH0.29 ± 0.020.29 ± 0.020.35 ± 0.040.52 ± 0.020.65 ± 0.09
GR536ΔfecA-E::kan ΔzupT::catNone0.09 ± 0.010.08 ± 0.020.17 ± 0.020.71 ± 0.010.22 ± 0.03
ΔmntH ΔfeoABC ΔentC
GR538ΔfecA-E::kan ΔzupT::cat ΔfeoABC ΔentCMntH0.52 ± 00.51 ± 0.010.51 ± 0.040.71 ± 0.010.81 ± 0
GR537ΔfecA-E::kan ΔmntH::cat ΔfeoABC ΔentCZupT0.40 ± 0.20.42 ± 0.020.64 ± 0.010.76 ± 0.010.79 ± 0.02
GR539ΔfecA-E::kan ΔfeoABC::cat ΔentCZupT, MntH0.50 ± 0.020.51 ± 0.050.68 ± 0.040.75 ± 0.020.83 ± 0.01
  • a Overnight cultures grown in Luria-Bertani broth were diluted 1:500 in minimal medium and grown overnight. Cells were diluted 1:500 in fresh minimal medium, and after 2 h, cells were diluted 1:500 into fresh medium with the indicated additives. The dry weight was determined after 16 h of incubation at 37°C with shaking. Experiments were performed independently in duplicate, and the average with deviation was calculated.

  • b All at a final concentration of 50 μM.