TABLE 5.

TTSS genes regulated by SadARS as determined by DNA microarray analysis

PA no.GenePredicted functionaFold change in expressionb
ΔsadRS::Gm/WT, biofilmTn5/WT
PA0044exoTToxin, ADP-ribosyltransferase3.4−3.8d
PA1690pscUTranslocation machinery2.0
PA1691pscTTranslocation machinery1.9
PA1692Translocation machinery3.7−2.0
PA1693pscRTranslocation machinery2.2
PA1694pscQTranslocation machinery3.2−1.7
PA1695pscPTranslocation machinery2.8
PA1696pscOTranslocation machinery4.1−1.3
PA1697ATP synthase2.4
PA1698popNOuter membrane protein2.4
PA1699Unknown2.5
PA1700Unknown2.8
PA1701Unknown4.0−1.8
PA1702Unknown1.6
PA1703pcrDUnknown2.7
PA1704pcrRTranscriptional regulator1.9
PA1705pcrGType III regulatory protein2.8−1.5
PA1706pcrVTranslocation machinery2.9c−3.3d
PA1707pcrHType III regulatory protein2.7c−4.2d
PA1708popBPore formation2.9c−4.0d
PA1709popDPore formation2.9−3.7
PA1710exsCTTSS regulator2.6−3.2
PA1711Unknown−1.7
PA1712exsBTTSS regulator1.6−1.6
PA1713exsATTSS regulator2.0−1.3d
PA1714exsDTTSS regulator2.8−2.0d
PA1715pscBTranslocation machinery3.1−1.5
PA1716pscCOuter membrane protein3.4−1.6
PA1717pscDTranslocation machinery2.5−1.5
PA1718pscETranslocation machinery3.1−3.2
PA1719pscFTranslocation machinery2.7c−2.9d
PA1720pscGTranslocation machinery2.1−1.8
PA1721pscHTranslocation machinery2.5−2.1
PA1722pscITranslocation machinery3.5c−2.4d
PA1723pscJTranslocation machinery2.6−1.3
PA1724pscKTranslocation machinery2.4
PA1725pscLTranslocation machinery2.3
PA2191exoYToxin, adenylate cyclase3.2−2.0
  • a Predicted functions based on data at www.Pseudomonas.com.

  • b Fold changes determined using the program CyberT, at a P value of <0.005.

  • c Confirmed by QRT-PCR on original array samples.

  • d Confirmed by QRT-PCR on original array samples plus two independent samples.