TABLE 1.

Bacterial strains and plasmids

Strain or plasmidRelevant characteristic(s)Reference or source
Strains
    E. coli
        XL1-Blue recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac[FproAB lacIqZΔM15 Tn10 (Tcr)]Stratagene
        DH5α recA1 endA1 hsdR17 supE44 gyrA96 relA1Δ(lacZYA-argF)U169 (φ80dlacZΔM15)Invitrogen
    A. tumefaciens
        A348A136 containing pTiA6NC 19
        A136Strain C58 cured of pTi plasmid 48
        DL8A136 chvE deletion, KmrThis study
Plasmids
    pBluescript II KS(+/−)Cloning vector, ColE1 AprStratagene
    pJZ45.1-kb fragment containing PvirB-lacZ-nptII cloned into pMON596, Inc P Specr 53
    pRG109 PN25-His6-virG in pJZ4, SpecrThis study
    pRG108 PN25-His6-virG(N54D) in pJZ4, SpecrThis study
    pYW47 PN25-His6-virG in pYW15, IncW Apr 47
    pAM13 PN25-His6-virG(N54D) in pYW15, IncW Apr 32
    pSM102 occQ::lacZ, IncP Apr 39
    pVRA8 virA from pTiA6 in pUCD2, pBR322ori, IncW Apr 25
    pMutA virA(G665D) in pUCD2, pBR322ori, IncW Apr 29
    pYW15Broad-host-range expression vector, IncW Apr 47
    pFF4 PN25-virA in pQE50, ColE1 AprThis study
    pFQ25 PN25-virA and PN25-virG, IncW AprThis study
    pYW21 PN25-His6-virA(285-829) in pYW15, Apr 45
    pYW39 PN25-His6-virA(285-829)(G665D) in pYW15, Apr 47
    pAM23 PN25-His6-virA(285-711) in pYW15, Apr 32
    pAM28 PN25-His6-virA(285-711) (G665D) in pYW15, Apr 32
    pRG100 PN25-His6-virA(426-829) (G665D) in pYW15, AprThis study
    pRG118 PN25-His6-virA(426-711) (G665D) in pYW15, AprThis study
    pRG119 PN25-His6-virA(426-711) in pYW15, AprThis study
    pRG120 PN25-His6-virA(426-829) in pYW15, AprThis study
    pRG135 virA(1-711) in pYW15, AprThis study
    pRG157 chvE from A136 in pUC19, ColE1 AprThis study
    pRG159Kmr gene cloned in BamH I site of chvE in pRG157, ColE1 Apr KmrThis study
    pRG162 virA(E210V) in pBluescript II KS (+/−), ColE1 AprThis study
    pRG165 PN25-chvE in pYW15, AprThis study
    pRG166 PN25-chvE(T211M) in pYW15, AprThis study
    pRG168 virA and PN25-chvE in pYW15, AprThis study
    pRG169 virA and PN25-chvE(T211M) in pYW15, AprThis study
    pRG170 virA(E210V) and PN25-chvE in pYW15, AprThis study
    pRG171 virA(E210V) and PN25-chvE(T211M) in pYW15, AprThis study