Oligonucleotide primers for construction of gfp fusion plasmids

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  • a The recognition sequence for the restriction endonuclease is underlined, and mismatched bases with respect to the wild-type B. subtilis sequence are in boldface type. The sites of the initiation codon and termination codon of each gene (the first four letters of the primer name correspond to the gene locus symbol) are marked with a box and thick underline, respectively.