TABLE 2.

Bacterial strains

StrainRelevant characteristic(s)Source and/or reference
B. subtilis
    168trpC2Laboratory stock
    SDB01168 psd::neo30
    SDB02168 pssA10::spc30
    SDB011168 Pspac-cdsAT. Yamamoto
    SDB012168 Pspac-yhdOT. Yamamoto
    SDB013168 Pspac-mprF/yfiXY. Sadaie
    SDB014168 mprF/yfiX::tetK. Misawa
    SDB110168 Pspac-pgsAH. Takahashi
    SDB206168 ywiE2::neo ywjE::spc clsA::pMutin422
    SDB1001168 amyE::(PcitM-gfp cat)This study
    SDB1006168 amyE::(Pxyl-gfp-pgsA spc)This study
    SDB1010168 amyE::(PcitM-pssA-gfp cat)This study
    SDB1012168 amyE::(PcitM-gpsA-gfp cat)This study
    SDB1014168 amyE::(Pxyl-gfp-psd spc)This study
    SDB1017168 amyE::(Pxyl-gfp-mprF spc)This study
    SDB1018168 amyE::(Pxyl-gfp-cdsA spc)This study
    SDB1019168 amyE::(Pxyl-gfp-yhdO spc)This study
    SDB1020168 amyE::(PcitM-ugtP-gfp cat)This study
    SDB1021168 amyE::(Pxyl-gfp-ugtP spc)This study
    SDB1022168 amyE::(Pxyl-gfp-dgkA spc)This study
    SDB1101168 amyE::(Pxyl-gfp-clsA spt)This study
    SDB1109168 amyE::(Pxyl-clsA+9-gfp spc)This study
    GS95168 gpsA::spcG. Schujman, 36
    KP261PY79 ugtP::neoK. Price, 46
    ASK510168 Pspac-ftsZK. Asai
    SDB1010F168 amyE::(PcitM-pssA-gfp cat) Pspac-ftsZThis study
    SDB1109F168 amyE::(Pxyl-clsA+9-gfp spc) Pspac-ftsZThis study
    ts1168 ts1(ftsZ1)K. Asai, 3
    SDB1010Tts1(ftsZ1) amyE::(PcitM-pssA-gfp cat)This study
    SDB1109Tts1(ftsZ1) amyE::(Pxyl-clsA+7-gfp spc)This study
    SDB1209168 clsA-gfp eryThis study
    SDB1220168 pssA-gfp eryThis study
E. coli
    MC4100F araD139 Δ(argF-lac)U169 rpsL150 relA1 flbB5301 deoC1 ptsF25 rbsRLaboratory stock
    W3110F λ IN(rrnD-rrnE)1 rph1Laboratory stock
    GN10W3110 ΔpssA10::cat48
    S107W3110 pssA1S. Nishijima, 39
    UE81ΔpssA10::cat ParaBAD-pssAThis study
    DH5supE44 hsdR17 recA1 gyrA96 thi-1 relA1Laboratory stock