TABLE 3.

Plasmids

PlasmidRelevant characteristicsSource and/or reference or primer pair used
pDHCMGFPbla amyE3 PcitM-gfp cat amyE5H. Yamamoto
pPssA-GFPpDHCMGFP PcitM-pssA-gfppssASph-pssAasNhe
pGpsA-GFPpDHCMGFP PcitM-gpsA-gfpgpsASph-gpsAasNhe
pUgtP-GFPpDHCMGFP PcitM-ugtP-gfpugtPSal-ugtPasNhe
pSG1154bla amyE3 spc Pxyl-gfp amyE5P. Lewis, 26
pClsA+9-GFPpSG1154 Pxyl-clsA+9-gfpclsAKpnh-clsAasClah
pSG1729bla amyE3 spc Pxyl-gfp amyE5P. Lewis, 26
pGFP-PgsApSG1729 Pxyl-gfp-pgsApgsAXho-pgsAasCla
pGFP-ClsApSG1729 Pxyl-gfp-clsAclsAClanm-clsAasEconm
pGFP-PsdpSG1729 Pxyl-gfp-psdpsdHind-psdasEco
pGFP-YfiX2pSG1729 Pxyl-gfp-mprFyfiXXho-yfiXasEco2
pGFP-CdsApSG1729 Pxyl-gfp-cdsAcdsAXho-cdsAasCla
pGFP-YhdOpSG1729 Pxyl-gfp-yhdOyhdOXho-yhdOasCla
pGFP-UgtPpSG1729 Pxyl-gfp-ugtPugtPBam-ugtPasClaN
pGFP-DgkApSG1729 Pxyl-gfp-dgkAdgkAXho-dgkAasEco
pMm2ery bla lacZ lacI Pspac gfpK. Asai, 56
pMPssA-GFPpMm2 ′pssA-gfppssASal-pssAasEco
pMClsAj-GFPpMm2 ′clsA-gfpclsASalj-clsAasEcoj