TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant genotype and propertySource or reference
E. coli strains
    W3110Wild typeLaboratory stock
    LW1W3110 Δcrp::KanThis study
    LW5W3110 Δ(lsrACDBFG)::KanThis study
    LW6W3110 ΔlsrR::KanThis study
    LW10W3110 ΔlsrK::KanThis study
    HT28W3110 Δcya::Kan29
    ZK126W3110 ΔlacU169 tna-211
    ZK1000ZK126 ΔrpoS::Kan8
    LW2ZK126 Δcrp::KanThis study
    LW7ZK126 ΔluxS::KanThis study
    LW8ZK126 ΔlsrR::KanThis study
    LW9ZK126 Δ(lsrACDBFG)::KanThis study
    LW11ZK126 ΔlsrK::KanThis study
V. harveyi strains
    BB152BB120 luxL::Tn5 (AI-1, AI-2+)52
    BB170BB120 luxN::Tn5 (sensor 1, sensor 2+)5
Plasmids
    pFZY1galK′-lacZYA transcriptional fusion vector, Apr32
    pLW10pFZY1 derivative, containing luxS promoter region, AprThis study
    pLW11pFZY1 derivative, containing lsrACDBFG promoter region, AprThis study
    pLW12pFZY1 derivative, containing mutated lsrACDBFG promoter region, AprThis study
    pYH10pFZY1 derivative, containing pfs promoter region, AprThis study
    pLW9pCR-Blunt (Invitrogen) derivative, containing luxS promoter region, KanrThis study
    pHA7EpBR322 derivative, crp+ Apr27
    pIT302pACYC184 derivative, cya+ Cmr29