TABLE 2.

Phenotypic switching of V. vulnificus 1003 phase variants

Parent strainaResulting phenotypes (avg % of total colonies)b
Opaque (SE)Translucent (SE)Rugose (SE)
1003(O)55.2 (23.0)30.6 (16.2)13.0 (3.0)
BG(T1)17.3 (12.4)74.7 (15.9)5.8 (2.9)
BG(T2)1.7 (0.9)94.3 (2.1)3.8 (2.2)
AZ(T)0.5 (0.4)98.1 (1.7)0 (0.0)
ABZ1(T)0 (0.0)78.2 (16.0)18.2 (12.8)
GMB4(T)7.9 (7.7)79.8 (10.5)8.3 (5.5)
BG(R), 30°C15.8 (3.2)0.1 (0.04)53.8 (2.8)
BG(R), 37°C26.3 (15.5)0 (0.0)53.1 (21.8)
ABZ1(R), 30°C0 (0.0)42.9 (21.0)42.0 (13.0)
ABZ1(R), 37°C0 (0.0)3.7 (3.7)94.2 (5.0)
  • a O, opaque; T, translucent; R, rugose. Switching assays were conducted for one to three cycles of culture passaging and plating as described in the text, with O and T broth cultures incubated at 34 to 35°C and plates incubated at 30°C; R broth cultures and plates were incubated at either 30 or 37°C as indicated.

  • b The total percentage of colonies for a given strain does not equal 100 because some colonies were sectored while others were of an intermediate phenotype.