TABLE 2.

Summary of protein-protein interactions involving X. axonopodis pv. citri T4SS components observed in this study

Bait name (residues in bait)Bait gene no.Total positive preys sequencedaSpecific preys [name or gene no: initial codons of preys (no. of times observed)]
Chromosomal T4SS
    VirD4 (1-557)XAC262368XAC0151: 11(1), 22(1), 25(1), 40(1), 120(1)
XAC1918: 120(1), 222(1), 229(1), 306(1), 360(1), 369(1), 470(2)
XAC3266: 301(1), 318(1), 320(2), 416(1), 514(1), 658(1), 663(1), 1)
XAC2609: 30(2), 90(1), 127(1), 144(1), 170(1), 187(1), 314(1)
XAC0096: 329(1), 330(1), 355(1), 402(1), 463(1), 478(1), 500(1)
XAC0574: 10(1), 75(1), 80(1), 82(1), 88(1), 96(1), 134(1)
XAC1165: 1(1), 6(1)
XAC0466: 40(1), 124(1), 158(1), 195(1), 227(1), 280(1), 308(1), 352(1), 400(2), 436(1), 456(1), 471(1)
XAC3634: 1(1), 13(1), 18(1), 20(1), 29(1), 38(1)
XAC4264: 94(1)
XAC0323: 30(1)
XAC2885: 82(1)
    XAC0466 (450-589)XAC04661cVirD4/XAC2623: 136(1)
    XAC2609 (1-431)XAC260911XAC2610: 19(1), 28(1), 39(1), 41(1), 49(2), 51(4), 76(1)
    XAC2610 (1-267)XAC26109FliK/XAC1949: 255(2), 265(3), 266(1)
XAC2609: 9(1)
VirB11/XAC2618: −14(1)
BfeA/XAC3207: 596(1)
    VirB11 (1-346)XAC261860VirB11/XAC2618: R(54),b 11(1)
YhjX/XAC2488: 343(2), 348(1)
PoxF/XAC4066: 80(1)
XAC3517: 651(1)
    XAC2622/VirB7 (1-139)XAC26226VirB9/XAC2620: 19(4), 101(1), 130(1)
pXAC64 T4SS
    VirB1 (1-292)XACb00366VirB9/XACb0039: 28(1), 30(3), 32(2)
    XACb0042 (33-131)XACb004213VirB6/XACb0041: −4(2), −3(4), −2(1), 2(1), 3(1), 4(1), 8(3)
    XACb0043 (1-82)XACb004314XACb0021b: 17(1), 20(1), 21(1), 25(1), 31(2), 33(1), 34(1)
TatC/XAC4216: 1(4)
FabB/XAC3625: 1(1)
RhlB/XAC3829: 359(1)
    XACb0035 (1-139)XACb003527XACb0035: R(11), −133(2), −99(1), −65(1), −55(1), 1(3), 2(4), 3(4)
    XACb0032 (1-107)XACb003211XACb0033: −46(1), −43(1), −4(1), 3(1), 5(2), 6(1), 8(1), 16(1), 18(1)
XAC1696: −50(1)
    XACb0025/TrwA (1-131)XACb00259XACb0025: R(5), −4(2), 2(2)
  • a Not including false-positive preys mentioned in the text and described in reference 1.

  • b R indicates preys derived from recombination events between the bait and prey vectors as described in text and in reference 1.

  • c Forty-six other preys were derived from TolC, a false-positive prey, when using this library (1).