TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidRelevant characteristic(s)Reference or source
Strains
    LF82E. coli isolated from an ileal biopsy of a CD patient14
    2E4Tn5phoA insertion into yfgL of strain LF827
    OA8Tn5phoA insertion into yfgL of strain LF827
    51F8Tn5phoA insertion into yfgL of strain LF827
    52D11Tn5phoA insertion into fimA of strain LF827
    LF82-ΔyfgLLF82 isogenic mutant with the yfgL gene deletedThis study
    MC4100E. coli K-12Laboratory stock
    MC4100-ΔyfgLMC4100 isogenic mutant with the yfgL gene deletedThis study
    E12860/0Enteroinvasive E. coli strain16
    E12860/0-ΔyfgLEnteroinvasive E. coli isogenic mutant with the yfgL gene deletedThis study
Plasmids
    pHSG575E. coli cloning vector, chloramphenicol resistant39
    pKOBEGpBAD cloning vector harboring λ phage redγβα operon, chloramphenicol resistant12
    pBAD33E. coli cloning vector, chloramphenicol resistant20
    pPBI01pHSG575 harboring the 11.2-kb SalI fragment with the entire fim operon of E. coli K-12 strain J967
    pPBI07pBAD33 harboring the 1.2-kb XbaI-PstI fragment with the entire yfgL gene of strain LF82This study