TABLE 2.

Germination of spores of various strains via the GerA receptora

StrainGenotypeCFU/12 hRate of DPA release (% of maximum value)
FB72ΔgerA ΔgerB ΔgerK1.5 × 105<2
PS8321.5 × 108104
PS3611ΔgerB ΔgerK4.1 × 107100b
PS3612gerACAla18 ΔgerB ΔgerK1.5 × 105<2
PS3629ΔamyE::(PgerA-gerACCys18) gerACAla18 ΔgerB ΔgerK1.4 × 10725
PS3630ΔamyE::(PgerA-gerACAla18) ΔgerB ΔgerK4.7 × 107104
PS3631ΔamyE::(PsspB-gerACCys18) gerACAla18 ΔgerB ΔgerK1.5 × 108360
PS3632ΔamyE::(PsspB-gerACAla18) ΔgerB ΔgerK7 × 10610
PS3633ΔamyE::(PsspB-gerACCys18) ΔgerB ΔgerK470
PS3660ΔamyE::(PsspB-gerBCCys20) ΔgerB ΔgerK3.4 × 1059
PS3661ΔamyE::(PsspB-gerBCAla20) ΔgerB ΔgerK4.2 × 107115
  • a Spores were prepared, cleaned, and tested for colony-forming ability and for DPA release upon germination with l-alanine as described in the text. CFU values are the averages of duplicate determinations with two independent spore preparations; individual values differed by ≤25% from the average value. DPA release rates are the averages of duplicate measurements with two independent spore preparations; individual values differed by ≤25% from the average values.

  • b This value was set at 100%.