TABLE 1.

B. subtilis strains used in this study

StrainGenotype and phenotypeaSource or reference
FB10gerBB1*18
FB72ΔgerA ΔgerB ΔgerK Cmr Spr Tcr19
PS832Wild typeLaboratory stock
PS3604gerBCCys20 Spr8
PS3605gerBCAla20 Spr8
PS3608ΔgerA Cmr8
PS3611ΔgerB ΔgerK Cmr Emr Spr8
PS3612gerACAla18 ΔgerB ΔgerK Cmr Emr Spr8
PS3629ΔamyE::(PgerA-gerACCys18) gerACAla18 ΔgerB ΔgerK Cmr Emr Kmr SprThis work
PS3630ΔamyE::(PgerA-gerACAla18) ΔgerB ΔgerK Cmr Emr Kmr SprThis work
PS3631ΔamyE::(PsspB-gerACCys18) gerACAla18 ΔgerB ΔgerK Cmr Emr Kmr SprThis work
PS3632ΔamyE::(PsspB-gerACAla18) ΔgerB ΔgerK Cmr Emr Kmr SprThis work
PS3633ΔamyE::(PsspB-gerACCys18) ΔgerB ΔgerK Cmr Emr Kmr SprThis work
PS3636ΔamyE::(PsspB-gerACAla18) gerBB1* KmrThis work
PS3637ΔamyE::(PsspB-gerACCys18) gerBB1* KmrThis work
PS3656gerBB1* gerBCCys20 SprThis work
PS3657gerBB1* gerBCAla20 SprThis work
PS3658ΔamyE::(PsspB-gerBCCys20) gerBB1* KmrThis work
PS3659ΔamyE::(PsspB-gerBCAla20) gerBB1* KmrThis work
PS3660ΔamyE::(PsspB-gerBCCys20) ΔgerB ΔgerK Cmr Emr Kmr SprThis work
PS3661ΔamyE::(PsspB-gerBCAla20) ΔgerB ΔgerK Cmr Emr Kmr SprThis work
PS3662ΔamyE::(PsspB-gerBCCys20) ΔgerA Cmr KmrThis work
PS3663ΔamyE::(PsspB-gerBCAla20) ΔgerA Cmr KmrThis work
PS3692ΔamyE::(PgerA-gerBCCys20) ΔgerA Cmr KmrThis work
PS3693ΔamyE::(PgerA-gerBCAla20) ΔgerA Cmr KmrThis work
PS3694ΔamyE::(PgerA-gerBCCys20) gerBB1* KmrThis work
PS3695ΔamyE::(PgerA-gerBCAla20) gerBB1* KmrThis work
  • a Cmr, resistance to chloramphenicol (5 μg/ml); Emr, resistance to erythromycin (1 μg/ml) and lincomycin (25 μg/ml); Kmr, resistance to neomycin (7 μg/ml); Spr, resistance to spectinomycin (100 μg/ml); Tcr, resistance to tetracycline (10 μg/ml).