TABLE 1.

Operon organizations and expression ratios of flagellar assembly genes in regions 1, 4, 5, and 6 and chemotaxis genes in regions 2 and 3

Gene ID no.aNamePutative functionExpression ratiob
AvgSDSig.
Region 1
    SO1529(−)motA-1Chemotaxis protein0.4700.184Yes
    SO1530(−)motB-1Chemotaxis protein0.4210.109Yes
Region 2
    SO2120(−)cheY-1Chemotaxis protein2.8991.570No
    SO2121(−)cheA-1Chemotaxis protein0.6590.216Yes
    SO2122(−)cheW-1Purine-binding chemotaxis protein0.4070.222Yes
    SO2123(−)Methyl-accepting chemotaxis protein1.0040.390No
    SO2124(−)cheR-1Chemotaxis protein methyltransferase0.9340.387No
    SO2125(−)cheD-1Chemotaxis protein0.8650.293No
    SO2126(−)cheB-1Protein-glutamate methylesterase0.8550.347No
Region 3
    SO2317(−)Methyl-accepting chemotaxis protein1.2950.590No
    SO2318(−)cheY-2Chemotaxis protein2.0851.680No
    SO2319Anti-anti-sigma factor, putative1.6001.127No
    SO2320(−)cheA-2Chemotaxis protein, interruption-N1.4141.222No
    SO2321(−)ISSod4, transposaseNo data
    SO2322(−)cheA-2Chemotaxis protein, interruption-C3.2991.611No
    SO2323(−)Methyl-accepting chemotaxis protein1.9331.425No
    SO2324(−)cheW-2Purine-binding chemotaxis protein0.8750.209Yes
    SO2325(−)cheR-2Chemotaxis protein methyltransferase1.1970.618No
    SO2326(−)cheD-2Chemotaxis protein, putative1.3730.666No
    SO2327(−)cheB-2Protein-glutamate methylesterase0.7130.266Yes
Region 4
    SO3202(−)cheW-3Purine-binding chemotaxis protein1.2470.495No
    SO3203(−)CheW domain protein0.9870.300No
    SO3204ParA family protein1.1030.368No
    SO3205Hypothetical protein1.2330.398No
    SO3206(−)cheB-3Protein-glutamate methylesterase1.6150.717No
    SO3207(+)Chemotaxis protein1.2260.387No
    SO3208(+)cheZChemotaxis protein1.7220.908No
    SO3209(+)cheY-3Chemotaxis protein1.8860.940No
    SO3210(+)fliARNA polymerase sigma-28 factor1.0520.331No
    SO3211(+)flhGFlagellar biosynthetic protein0.7490.141No
    SO3212(+)flhFFlagellar biosynthetic protein0.6320.293Yes
    SO3213(+)flhAFlagellar biosynthesis protein0.5580.149Yes
Region 5
    SO3936(+)motXSodium-type flagellar protein0.7880.323No
Region 6
    SO4286(+)motB-2Chemotaxis motB protein0.7370.328No
    SO4287(+)motA-2Chemotaxis motA protein0.6460.135Yes
Region 4
    SO3214Hypothetical protein0.3690.154Yes
    SO3215(+)flhBFlagellar biosynthetic protein0.6780.237Yes
    SO3216(+)fliRFlagellar biosynthetic protein0.4390.159Yes
    SO3217(+)fliQFlagellar biosynthetic protein0.6720.252No
    SO3218(+)fliPFlagellar biosynthetic protein0.5370.140Yes
    SO3219(+)fliOFlagellar protein0.5320.210Yes
    SO3220(+)fliNFlagellar motor switch protein0.5750.138Yes
    SO3221(+)fliMFlagellar motor switch protein0.7160.363No
    SO3222(+)fliLFlagellar protein0.7410.156Yes
    SO3223(+)fliKFlagellar hook-length control protein0.3780.244Yes
    SO3224(+)fliJFlagellar protein0.3280.168Yes
    SO3225(+)fliIFlagellum-specific ATP synthase0.5770.161Yes
    SO3226(+)fliHFlagellar assembly protein1.0170.868No
    SO3227(+)fliGFlagellar motor switch protein0.8390.401No
    SO3228(+)fliFFlagellar M-ring protein0.5840.132Yes
    SO3229(+)fliEFlagellar hook-basal body complex0.2840.127Yes
    SO3230(+)flrCFlagellar regulatory protein C0.3520.137Yes
    SO3231(+)flrBFlagellar regulatory protein B0.2000.073Yes
    SO3232(+)flrAFlagellar regulatory protein A0.3880.150Yes
    SO3233(+)fliSFlagellar protein0.5270.183Yes
    SO3234Hypothetical protein0.4970.273Yes
    SO3235(+)fliDFlagellar hook-associated protein0.5550.304Yes
    S03236(+)flaGFlagellin0.3450.078Yes
    SO3237(+)Flagellin0.3170.097Yes
    SO3238(+)Flagellin0.8460.471No
    SO3239(+)flgLFlagellar hook-associated protein0.4950.226Yes
    SO3241(+)flgJFlagellar protein0.4220.216Yes
    SO3242(+)flgIFlagellar P-ring protein0.3830.229Yes
    SO3243(+)flgHFlagellar L-ring protein0.4270.170Yes
    SO3244(+)flgGFlagellar basal-body rod protein0.3890.175Yes
    SO3245(+)flgFFlagellar basal-body rod protein0.3200.188Yes
    SO3246Hypothetical protein0.4340.413No
    SO3247(+)flgEFlagellar hook protein0.2690.136Yes
    SO3248(+)flgDBasal-body rod modification protein0.2760.066Yes
    SO3249(+)flgCFlagellar basal-body rod protein0.3000.109Yes
    SO3250(+)flgBFlagellar basal-body rod protein0.1420.053Yes
    SO3251(+)cheR-3Chemotaxis protein methyltransferase0.6090.188Yes
    SO3252(+)cheVChemotaxis protein0.5560.113Yes
    SO3253(−)Flagellar basal-body P-ring protein0.2570.071Yes
    SO3254(−)flgMRegulator of flagellin synthesis0.3070.164Yes
    SO3255(−)flgNFlagellar biosynthetic protein0.1900.086Yes
  • a + and − represent the operon location on the positive or negative strand, respectively. Some non-motility-related genes are also included in the table to keep the entity of a complete operon.

  • b The average expression ratio of the salted sample to the control was calculated from 12 replicates together with the respective standard deviation (SD), a P = 0.05 standard t test result (significant [Sig.]).